Transforming growth factor-beta stimulates the expression of fibronectin and collagen and their incorporation into the extracellular matrix.
Transforming growth factor-beta stimulates the expression of fibronectin and collagen and their incorporation into the extracellular matrix.
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发表时间：
1986-03
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通讯作者：
R. Ignotz;J. Massagué
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作者：
R. Ignotz;J. Massagué
我们已经研究了细胞外基质是否是转化生长因子β（TGF β）的生化靶点。我们发现TGF β增加了主要的细胞外基质蛋白、纤连蛋白和胶原蛋白的表达。这种效应是对TGF β的一般反应，见于原代培养物和来自各种类型（正常和转化）的已建立细胞系。纤连蛋白和胶原蛋白相对结合到基质中也响应于TGF β而增加。TGF β对鸡胚成纤维细胞中纤连蛋白水平的影响是快速的、选择性的、持久的和特异性的，并且涉及转录事件;它不被测试的其他生长因子模仿。TGF β诱导正常成纤维细胞的锚定非依赖性生长被纤连蛋白模拟，并被与其细胞表面受体结合的纤连蛋白抑制剂特异性阻断。结果表明纤连蛋白介导对TGF β的细胞反应的功能参与，并提出了基于靶细胞中细胞外基质控制的TGF β作用模型。
We have examined whether the extracellular matrix is a biochemical target for transforming growth factor-beta (TGFbeta). We find that TGFbeta increases the expression of the major extracellular matrix proteins, fibronectin and collagen. This effect is a general response to TGFbeta seen in primary cultures and established lines of cells from various types, normal and transformed. The relative incorporation of fibronectin and collagen into the matrix also increases in response to TGFbeta. The effect of TGFbeta on fibronectin levels as characterized in chick embryo fibroblasts is rapid, selective, persistent, and specific, and involves transcriptional events; it is not mimicked by other growth factors tested. The induction of anchorage-independent growth of normal fibroblasts by TGFbeta is mimicked by fibronectin and is specifically blocked by inhibitors of fibronectin binding to its cell surface receptor. The results demonstrate a functional involvement of fibronectin in mediating cellular responses to TGFbeta, and suggest a model for TGFbeta action based on the control of the extracellular matrix in target cells.