A palm-sized surface plasmon resonance sensor with microchip flow cell
A palm-sized surface plasmon resonance sensor with microchip flow cell
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DOI：
10.1016/j.talanta.2006.02.068
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发表时间：
2006-09-15
期刊：
影响因子：
6.1
通讯作者：
Hemmi, Akihide
中科院分区：
文献类型：
--
作者：
Nakajima, Hizuru;Harada, Yuuki;Hemmi, Akihide
为了提高 SPR 检测器对低分子量化合物的灵敏度，开发了一种带有微芯片流通池的小型表面等离子共振 (SPR) 传感器。这种便携式差分SPR检测器由一个LED、两个柱面透镜、一个圆形棱镜、一个分光镜、一个CCD、电子器件和一个具有两个流路（10毫米长、1毫米宽、20-100微米深）的聚二甲基硅氧烷/金微芯片组成。 3-巯基丙基三甲氧基硅烷用于密封微芯片。使用蔗糖和 IgA 评估现场定向 SPR 探测器的性能。 SPR强度出现剧烈变化。流动池的深度与SPR强度成反比。与尺寸为 10 mm（长）x 1 mm（宽）x 1 mm（深）的传统流通池相比，其对 10% 蔗糖和 0.9 nM IgA 的灵敏度分别提高了约 11 倍和 39 倍。这种现象似乎是由于SPR传感器上的物质因其尺寸效应而增加所致。这些结果表明，应用微芯片传感器进行SPR测量有可能提高低分子物质的SPR强度。 (c) 2006 Elsevier B.V. 保留所有权利。
A small-sized surface plasmon resonance (SPR) sensor with a microchip flow cell has been developed for the purpose of enhancing the sensitivity of the SPR detector for low molecular weight compounds. This portable differential SPR detector consisted of an LED, two cylindrical lenses, a round prism, a divided mirror, a CCD, electronics, and a polydimethylsiloxane/gold microchip with two flow paths (10 mm long, 1 mm wide, 20-100 mu m deep). 3-Mercaptopropyltrimethoxysilane was used for sealing the microchip. The performance of the on-site orientated SPR detector was estimated using sucrose and IgA. A drastic change in the SPR intensity appeared. The depth of the flow cell was in inverse proportion to the SPR intensity. Compared to a conventional flow cell having the size of 10 mm (L) x 1 mm (W) x 1 mm (D), its sensitivity to 10% sucrose and 0.9 nM IgA increased about 11 and 39 times, respectively. This phenomenon seemed to be due to the increase in the substance on the SPR sensor based on its size effect. These results showed that the application of the microchip sensor for SPR measurement has the possibility for improvement of the SPR intensity for low molecular substances. (c) 2006 Elsevier B.V. All rights reserved.