A CELL-CYCLE NUCLEAR AUTOANTIGEN CONTAINING WD-40 MOTIFS EXPRESSED MAINLY IN S-PHASE AND G(2)-PHASE CELLS
A CELL-CYCLE NUCLEAR AUTOANTIGEN CONTAINING WD-40 MOTIFS EXPRESSED MAINLY IN S-PHASE AND G(2)-PHASE CELLS
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DOI：
10.1006/bbrc.1995.1288
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发表时间：
1995-02-27
影响因子：
3.1
通讯作者：
TAN, EM
中科院分区：
文献类型：
--
作者：
MURO, Y;CHAN, EKL;TAN, EM
最近，使用来自癌症患者的自身抗体表征了主要在S和G（2）期细胞中表达的新型核蛋白（Exp. Cell Res.，212，255-261，1994），通过免疫筛选分离cDNA克隆，序列分析显示cDNA克隆编码713个氨基酸残基的多肽，其暂时命名为SG 2NA（S/G（2）核抗原）。在SG 2NA的羧基端有6个拷贝的WD-40基序，是一个大家族蛋白的特征，其原型是β-转导蛋白/分裂增强子（TLE），此外，还有与TLE蛋白相关的核定位序列和磷酸化位点。在某些癌症患者中自发发生的自身抗体已经成为用于鉴定细胞蛋白质的有用试剂，这是第一次报道一种新的WD-40蛋白，其是自身免疫应答的靶点。(C)出版社：Academic Press
Recently a novel nuclear protein mainly expressed in S and G(2) phase cells was characterized using autoantibodies from a cancer patient (Exp. Cell Res., 212, 255-261, 1994), cDNA clones were isolated by immunoscreening, and sequence analysis revealed that the cDNA clones encoded a 713-amino acid residue polypeptide which is provisionally named SG2NA (S/G(2) nuclear antigen). In the carboxyl terminal half of SG2NA, there are 6 copies of WD-40 motifs characteristic of a large family of proteins of which the prototype is beta-transducin/enhancer of split (TLE), In addition, there are nuclear localization sequence and phosphorylation sites related to the TLE proteins. Autoantibodies occurring spontaneously in certain cancer patients have been useful reagents for identifying cellular proteins and this is the first report of a novel WD-40 protein which is the target of an autoimmune response. (C) 1995 Academic Press, Inc.