Molecular genetic characterization of XRCC4 function
Molecular genetic characterization of XRCC4 function
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DOI：
10.1093/intimm/9.10.1607
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发表时间：
1997-10-01
影响因子：
4.4
通讯作者：
Alt, FW
中科院分区：
文献类型：
--
作者：
Mizuta, R;Cheng, HL;Alt, FW
XRCC4是一种普遍表达的334个氨基酸的蛋白，参与DNA双链断裂的修复和V(D)J重组，但其功能尚不清楚。在本研究中，我们使用突变方法和酵母双杂交方法对该蛋白质进行了初步表征，我们证明了 XRCC4 蛋白位于细胞核中，我们还证明了在瞬时 V(D)J 重组测定中 XRCC4 功能不需要几个潜在的磷酸化位点，此外，我们证明 XRCC4 在体内形成同二聚体，同二聚化结构域位于氨基酸 115-204 内。最后，我们定义了 XRCC4 的核心结构域，该结构域在 V(D)J 重组中发挥作用，包含氨基酸 18-204，讨论了 XRCC4 的潜在功能。
XRCC4 is a generally expressed protein of 334 amino acids that is involved in the repair of DNA double-strand breaks and in V(D)J recombination, but its function is unknown. In this study, we have used a mutational approach and the yeast two-hybrid method to perform an initial characterization of this protein, We show that the XRCC4 protein is located in the nucleus, We also demonstrate that several potential phosphorylation sites are not required for XRCC4 function in a transient V(D)J recombination assay, In addition, we show that XRCC4 forms a homodimer in vivo with the homodimerization domain being located within amino acids 115-204. Finally, we define a core domain of XRCC4 that functions in V(D)J recombination and comprises amino acids 18-204, Potential functions of XRCC4 are discussed.