Molecular analysis of a novel alkaline metal salt (NaCl)-responsive WRKY transcription factor gene IlWRKY1 from the halophyte Iris lactea var. chinensis
Molecular analysis of a novel alkaline metal salt (NaCl)-responsive WRKY transcription factor gene IlWRKY1 from the halophyte Iris lactea var. chinensis
复制标题
来自盐生植物 Irislacteavar. 的新型碱金属盐 (NaCl) 响应 WRKY 转录因子基因 IlWRKY1 的分子分析。
DOI：
10.1016/j.ibiod.2017.11.021
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发表时间：
2018
影响因子：
4.8
通讯作者：
Huang S. Z.
中科院分区：
文献类型：
--
作者：
Tang J.;Liu Q. Q.;Yuan H. Y.;Zhang Y. X.;Huang S. Z.
土壤盐碱化限制了植物生长，威胁到作物生产。研究植物，特别是盐生植物对盐的反应是十分必要的。WRKY转录因子（TFs）在植物碱金属（钠）盐胁迫响应中具有重要的调控作用。虹膜lacteavar。一种观赏多年生草本盐生植物。本研究首次从i中克隆到一个新的WRKY TF基因ilwrky1。lacteavar.chinensis。IlWRKY1cDNA序列包含一个1083 bp的开放阅读框（ORF），编码361个氨基酸的蛋白（Mw: 38.77 kDa; pI: 5.22）。蛋白序列保守域分析表明，IlWRKY1属于ⅢWRKY tf族。系统发育分析表明，IlWRKY1与masparagus officinalisa的AoWRKY41和musa acuminatassubsp .malaccensis的MaWRKY41更为接近。此外，对mRNA转录水平的分析显示，NaCl胁迫下，ofilwrky1的表达显著增加。lacteavar。在NaCl胁迫24 h后达到最高水平，表明atilwrky1可能参与了胁迫。lacteavar。钠盐反应。本研究将有助于研究植物耐钠性调控的分子机制。lacteavar.chinensisbyIlWRKY1。
Soil salinization limits plant growth and threatens crop production. Research on salt response in plants, especially halophytes, is imperative. WRKY transcription factors (TFs) have critical regulatory roles in plant alkaline metal (sodium) salt stress response.Iris lacteavar.chinensisis an ornamental and perennial herbaceous halophyte. In this study, a new WRKY TF geneIlWRKY1was firstly cloned fromI. lacteavar.chinensis.IlWRKY1cDNA sequence contains an open reading frame (ORF) of 1083 bp, which encodes a protein (Mw: 38.77 kDa; pI: 5.22) of 361 amino acids. Analysis of the conserved domains of protein sequence indicated that IlWRKY1 belonged to the Group Ⅲ WRKY TFs. Phylogenetic analysis showed that the IlWRKY1 was much closer to AoWRKY41 fromAsparagus officinalisand MaWRKY41 fromMusa acuminatasubsp.malaccensis. Furthermore, analysis of mRNA transcriptional levels revealed that the expression ofIlWRKY1was notably increased under NaCl stress inI. lacteavar.chinensisshoots, and achieved the highest level after 24 h of NaCl stress, suggesting thatIlWRKY1may be involved inI. lacteavar.chinensissodium salt responses. This work will conduce to the research on the molecular mechanism underlying the regulation of the sodium salt tolerance inI. lacteavar.chinensisbyIlWRKY1.