MICROMACHINING OF CAPILLARY ELECTROPHORESIS INJECTORS AND SEPARATORS ON GLASS CHIPS AND EVALUATION OF FLOW AT CAPILLARY INTERSECTIONS
MICROMACHINING OF CAPILLARY ELECTROPHORESIS INJECTORS AND SEPARATORS ON GLASS CHIPS AND EVALUATION OF FLOW AT CAPILLARY INTERSECTIONS
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DOI：
10.1021/ac00073a029
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发表时间：
1994-01-01
影响因子：
7.4
通讯作者：
HARRISON, DJ
中科院分区：
文献类型：
--
作者：
FAN, ZH;HARRISON, DJ
采用微机械加工技术将毛细管电泳进样器集成在1 × 2cm的平板玻璃芯片上。在4 s内实现了三种氨基酸的分离。在进样点和检测点之间为470 V时，获得最多6800个理论塔板。2500 V/cm的电场和2.3 W/m的功率耗散不会导致焦耳加热效应，这是由于玻璃芯片的热质量和传导性。发现通道交叉点处的泄漏是由对流和扩散效应引起的。提出了器件制造的方法。
A capillary electrophoresis system with a sample injector has been integrated on a 1 X 2 cm planar glass chip by use of micromachining techniques. Separation of three amino acids was achieved in the device within 4 s. A maximum of 6800 theoretical plates was obtained with 470 V between injection and detection points. Electric fields of 2500 V/cm and power dissipation of 2.3 W/m did not result in Joule heating effects due to the thermal mass and conductivity of the glass chips. Leakage at channel intersection points was found to arise from convective as well as diffusion effects. Methods of device fabrication are presented.