Structural Characterization and α-Glucosidase Inhibitory and Antioxidant Activities of Fucoidans Extracted from Saccharina japonica
Structural Characterization and α-Glucosidase Inhibitory and Antioxidant Activities of Fucoidans Extracted from Saccharina japonica
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DOI：
10.1002/cbdv.202000233
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发表时间：
2020-07-07
影响因子：
2.9
通讯作者：
Wang, Hong
中科院分区：
文献类型：
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作者：
Ke, Songze;Wei, Bin;Wang, Hong
从海带中提取两个硫酸化褐藻糖胶组分（Lj 3和Lj 5），然后进行酸水解，得到Lj 3 h和Lj 5 h。Lj 3 h和Lj 5 h使用IR、甲基化分析和质谱进行表征。发现Lj 3 h和Lj 5 h是均一的低分子量岩藻聚糖。具体地，Lj 3 h由在C-2和/或C-4处具有硫酸根的1，3-连接的α-L-吡喃岩藻糖残基的主链和在岩藻糖残基的C-2和/或C-4处分支的三种不同的单糖（半乳糖、葡萄糖、甘露糖）组成。Lj 5 h含有通过1 -> 3键（岩藻半乳聚糖）和1 -> 6键连接的半乳聚糖连接的交替的吡喃半乳糖残基和吡喃岩藻糖残基的主链。硫酸化模式主要位于岩藻糖或半乳糖残基的C2/C4位，岩藻糖残基的C4位和半乳糖残基的C2、C3或/和C6位分支较多。体外实验表明，在测试的四种岩藻聚糖中，只有Lj 5显示出有效的α-葡萄糖苷酶抑制活性，IC 50为153.27 +/- 22.89 μ g/mL，两种亲本岩藻聚糖Lj 3和Lj 5显示出比其衍生物更好的抗氧化活性。这些发现突出了日本海蛞蝓衍生的岩藻多糖的结构和生物活性的多样性。
Two sulfated fucoidan fractions (Lj3 and Lj5) were extracted from Saccharina japonica and then subjected to acid hydrolysis to obtain Lj3h and Lj5h. Lj3h and Lj5h were characterized using IR, methylation analysis, and mass spectrometry. It was found that Lj3h and Lj5h were homogeneous low molecular weight fucoidans. Specifically, Lj3h was composed of the main chain of 1,3-linked alpha-L-fucopyranose residues with sulfate at C-2 and/or C-4 and three different monosaccharides (galactose, glucose, mannose) branched at C-2 and/or C-4 of fucose residue. Lj5h contained backbones of alternating galactopyranose residues and fucopyranose residues attached via a 1 -> 3 linkage (galactofucan) and 1 -> 6 linked galactan. The sulfation pattern was mainly located at C2/C4 fucose or galactose residues and more branches occupied at C-4 of fucose residue and C-2, C-3 or/and C-6 of galactose residue. In vitro assay indicated that, among the four fucoidans tested, only Lj5 showed potent alpha-glucosidase inhibitory activity with IC50 of 153.27 +/- 22.89 mu g/mL, and the two parent fucoidans, Lj3 and Lj5, showed better antioxidant activity than their derivatives. These findings highlight the structure and bioactivity diversity of Saccharina japonica-derived fucoidans.