Induced genetic ablation of Rest leads to the alteration of stimulus‐induced response of the vagal nerve
Induced genetic ablation of Rest leads to the alteration of stimulus‐induced response of the vagal nerve
复制标题
诱导的休息基因消融导致迷走神经刺激诱导反应的改变
DOI：
10.1111/gtc.12819
复制
发表时间：
2021
期刊：
影响因子：
2.1
通讯作者：
Kunisada Takahiro
中科院分区：
文献类型：
--
作者：
Aoki Hitomi;Abe Chikara;Hara Akira;Miyazaki Tatsuhiko;Morita Hironobu;Kunisada Takahiro
Rest（RE 1沉默转录因子，也称为Nrsf）通过阻止神经元基因的早熟表达参与维持神经元干/祖细胞的未分化状态。为了进一步研究Restin神经元的功能，我们通过产生Restconditional knockout小鼠来产生和检查在神经组织中特异性地诱发Restin基因消融的小鼠。由于Restknockout小鼠是胚胎致死的，我们使用Sox 1-Creallele从神经细胞分化的早期阶段（包括神经嵴衍生的神经细胞）切除了被抑制的Restgene。使用这种条件性RestknockoutSox 1-Cre; Restflox/mouse小鼠，我们揭示了Restin在胃和心脏副交感神经系统中的作用。
Rest (RE1‐silencing transcription factor, also called Nrsf) is involved in the maintenance of the undifferentiated state of neuronal stem/progenitor cells by preventing precocious expression of neuronal genes. In order to further investigate the function ofRestin neurons, we generated and examined mice evoking genetic ablation ofRestspecifically in neural tissues by generatingRestconditional knockout mice. As theRestknockout mice are embryonically lethal, we used aSox1‐Creallele to excise the floxedRestgene from the early stage of nerve cell differentiation including neural crest‐derived nerve cells. Using this conditionalRestknockoutSox1‐Cre; Restflox/floxmice, we have revealed the role ofRestin the parasympathetic nervous system in the stomach and heart