Temporal expression patterns of diapause-associated genes in flesh fly pupae from the onset of diapause through post-diapause quiescence
Temporal expression patterns of diapause-associated genes in flesh fly pupae from the onset of diapause through post-diapause quiescence
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DOI：
10.1016/j.jinsphys.2004.11.009
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发表时间：
2005-06-01
影响因子：
2.2
通讯作者：
Denlinger, DL
中科院分区：
文献类型：
--
作者：
Hayward, SAL;Pavlides, SC;Denlinger, DL
与蛹滞育相关的基因的时间表达模式的明显差异，指出在肉蝇，麻蝇crassipalpis。观察到的第一个变化是蛹化后2天（蛹达到滞育的显形阶段特征前1天）编码热休克蛋白90（HSP 90）的基因表达下降。与此相反，hsp 23和hsp 70转录检测不到的非滞育样品和d1-d4滞育程序化蛹，但上调后，滞育开始后，5天后pupariation。滞育蛹的甘油含量在滞育开始时也有所增加。编码增殖细胞核抗原（pcna）的基因被滞育下调，这发生在两个阶段，第一次下降表达后7天，蛹化第二次下降表达水平在第14天。蛹在20 ℃保温20天后转入10 ℃，在低温下滞育90-100天后结束。然而，蛹仍然处于滞育后的静止状态（d100-d150），并持续滞育样的hsp和pcna表达模式，直到成虫发育开始。甘油浓度和生存下降滞育后阶段。这项研究表明，在滞育开始时的基因表达模式中的一个独特的序列，但我们所监测的基因并不有助于从滞育到滞育后静止的过渡转换到隐蔽的发展潜力。(c)2004爱思唯尔有限公司保留所有权利。
Distinct differences in the temporal expression patterns of genes associated with pupal diapause were noted in the flesh fly, Sarcophaga crassipalpis. The first change observed was a decline in expression of the gene encoding heat shock protein 90 (hsp90) 2 days after pupariation (1 day before the pupa reaches the phanerocephalic stage characteristic of diapause). In contrast, hsp23 and hsp70 transcripts were undetectable in nondiapause samples and d1-d4 diapause-programmed pupae, but were up-regulated just after the start of diapause, 5 days after pupariation. An increase of glycerol content in diapausing pupae was also noted at the start of diapause. The gene encoding proliferating cell nuclear antigen (pcna) was diapause down-regulated, and this occurred in two phases, with the first decline in expression 7 days after pupariation and a second decline in the level of expression on day 14. For pupae held at 20 C for 20 days and transferred to 10 C, diapause ended after 90-100 days at the lower temperature. However, pupae remained in a state of post-diapause quiescence (d100-d150) and sustained diapause-like hsp and pcna expression patterns until adult development was initiated. Glycerol concentrations and survival declined during the post-diapause phase. This study suggests a distinct sequence in the pattern of gene expression at the onset of diapause, but the genes we have monitored do not contribute to the switch to covert developmental potential at the transition from diapause to post-diapause quiescence. (c) 2004 Elsevier Ltd. All rights reserved.