In vitro and in vivo evaluation of the antibacterial properties of a nisin-grafted hydrated mucin multilayer film
In vitro and in vivo evaluation of the antibacterial properties of a nisin-grafted hydrated mucin multilayer film
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DOI：
10.1016/j.polymertesting.2016.12.006
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发表时间：
2017-02
期刊：
影响因子：
5.1
通讯作者：
Xu-zhong Xu-Xu-zhong-Xu-5366709;Tingwei Jin;Binjun Zhang;Huihua Liu;Zi Ye;Qingwen Xu;Hao Chen;Bailiang Wang
中科院分区：
文献类型：
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作者：
Xu-zhong Xu-Xu-zhong-Xu-5366709;Tingwei Jin;Binjun Zhang;Huihua Liu;Zi Ye;Qingwen Xu;Hao Chen;Bailiang Wang
微生物感染是医疗器械领域，特别是植入物领域最严重的问题之一。在此，我们设计并构建了一种（粘蛋白/聚（乙烯亚胺））n（（粘蛋白/PEI）n）多层膜，采用逐层自组装方法和抗菌肽的接枝，以提高杀菌效果。水接触角测量和原子力显微镜图像显示，水合多层膜形成了一个高度亲水性的低粗糙度表面。功能化聚二甲基硅氧烷（PDMS）表面具有抑制牛血清白蛋白（BSA）吸附和减少细菌粘附的作用。（mucin/PEI）n-nisin涂层的杀菌活性，通过扫描电镜和活/死细菌活力试剂盒检测，具有显著的效果。由于嫁接了乳酸链球菌素而产生金黄色。在新西兰大白兔体内皮下切开，植入多层膜修饰和未涂覆的PDMS。创面外观评价和组织病理学分析均表明，抗菌涂层修饰的PDMS可促进创面愈合，并具有抗炎作用。该多层膜对人体晶状体上皮细胞无毒，可广泛应用于生物医学植入物的修饰。
Microbial infection is one of the most serious problems in the field of medical devices, particularly in implants. Herein, we have designed and constructed a (mucin/poly(ethyleneimine))n((mucin/PEI)n) multilayer film using a layer-by-layer self-assembly method and the grafting of antimicrobial peptides to enhance the bactericidal efficacy. Water contact angle measurements and atomic force microscopy images revealed that the hydrated multilayer film created a highly hydrophilic surface with a low roughness. The functionalized polydimethylsiloxane (PDMS) surfaces were shown to be effective in preventing bovine serum albumin (BSA) adsorption and in reducing bacterial adhesion. Bactericidal activity of the (mucin/PEI)n-nisin coatings, measured by scanning electron microscopy and a LIVE/DEAD bacterial viability kit, was remarkably effective againstS. aureusowing to the grafting of nisin.In vivosubcutaneous incisions were made in New Zealand white rabbits and were implanted with multilayer-film-modified and uncoated PDMS. Both the evaluation of the appearance of the wound and the histopathology analysis demonstrated that implantation of the antibacterial-coating-modified PDMS promoted wound healing and showed an anti-inflammatory effect. The multilayer film proved to be nontoxic towards human lens epithelial cells, which can potentially be widely used to modify biomedical implants.