A NOVEL METHOD TO STUDY THE ELECTRODYNAMIC BEHAVIOR OF ACTIN-FILAMENTS - EVIDENCE FOR CABLE-LIKE PROPERTIES OF ACTIN
A NOVEL METHOD TO STUDY THE ELECTRODYNAMIC BEHAVIOR OF ACTIN-FILAMENTS - EVIDENCE FOR CABLE-LIKE PROPERTIES OF ACTIN
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DOI：
10.1016/s0006-3495(93)81188-3
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发表时间：
1993-10-01
影响因子：
3.4
通讯作者：
CANTIELLO, HF
中科院分区：
文献类型：
--
作者：
LIN, EC;CANTIELLO, HF
肌动蛋白是最丰富的细胞内蛋白质之一，在溶液中形成长线性聚电解质聚合物。一种新的技术，以处理单肌动蛋白丝在溶液中，允许的离子电流引起的研究沿着表面的电刺激肌动蛋白丝。在高（100 mM KCl）和低（1 mM KCl）离子强度条件下观察到聚合物表面的电流。这些数据与围绕肌动蛋白丝的反离子云的动态行为一致，所述肌动蛋白丝在电刺激时支持离子沿其纵轴沿着运动。反离子波在本质上是高度非线性的，并且在肌动蛋白丝的电刺激停止后保持很长时间。因此，在这份报告中，我们证明了肌动蛋白丝可以作为生物“电线”，因此可以被概念化为非线性非均匀传输线。肌动蛋白丝传导电信号的能力可能在细胞内信号的耦合中具有重要意义。
Actin, one of the most abundant intracellular proteins, forms long linear polyelectrolytic polymers in solution. A novel technique to handle single actin filaments in solution was developed that allows the study of ionic currents elicited along the surface of electrically stimulated actin filaments. Electrical currents were observed about the polymer's surface under both high (100 mM KCl) and low (1 mM KCl) ionic strength conditions. The data are consistent with a dynamic behavior of the counterionic cloud surrounding the actin filaments that support ionic movements along their longitudinal axis upon electrical stimulation. Counterionic waves were highly nonlinear in nature and remained long after the electrical stimulation of the actin filaments ceased. In this report therefore, we demonstrate that actin filaments can function as biological ''electrical wires'' and can thus be conceptualized as nonlinear inhomogeneous transmission lines. This ability of actin filaments to conduct electrical signals may have important implications in the coupling of intracellular signals.