Over-expression of ZmPti1 , a homologue to Pti1 , increases salt tolerance of Arabidopsis thaliana
Over-expression of ZmPti1 , a homologue to Pti1 , increases salt tolerance of Arabidopsis thaliana
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DOI：
10.5897/ajb09.1660
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发表时间：
2010-02
影响因子：
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通讯作者：
H. Zou;Zhong-Yi Wu;Xiuhai Zhang;Yongqin Wang;Conglin Huang
中科院分区：
文献类型：
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作者：
H. Zou;Zhong-Yi Wu;Xiuhai Zhang;Yongqin Wang;Conglin Huang
研究表明Pti1在植物抗病途径中发挥着重要作用。然而，Pti1在盐胁迫条件下的作用尚未被研究。前期研究表明，玉米ZmPti1是由水杨酸（SA）、低温、甘露醇和盐诱导的。为了进一步分析ZmPti1的生物学功能，在拟南芥中过表达ZmPti1。在盐胁迫下，与野生型相比，转基因植物生长更好，具有更高的幼苗鲜重和干重（FW、DW）、种子产量和超氧化物歧化酶（SOD）活性； S1-1、S2-1转基因植株的幼苗FW和DW较WT显着增加； S1-1、S2-1转基因植株种子重量分别增加71%和106%；然而，转基因植物的丙二醛（MDA）含量和相对电导率（离子泄漏）保持在相对较低的水平。据目前所知，这是第一篇表明Pti1-like基因的过度表达增强植物耐盐性的报道。
Studies have shown that Pti1 plays an important role in plant disease resistance pathway. However, Pti1 have not been studied for its roles under salt stress condition. Previous study has shown that maize ZmPti1 is induced by salicylic acid (SA), low-temperature, mannitol and salt. In order to analyze the further biological functions of ZmPti1, ZmPti1 was over-expressed in Arabidopsis. Under salt stress, compared to wild type, transgenic plants grew better, had higher seedling fresh and dry weight (FW, DW), seed yields and the superoxide dismutase (SOD) activity; Seedling FW and DW of S1-1, S2-1 transgenic plants increased significantly than that of WT; Seed weight of S1-1, S2-1 transgenic plants increased 71 and 106% respectively; However malondialdehyde (MDA) content and relative electric conductivity (ion leakage) of transgenic plants were kept to a relative lower level. Based on the present knowledge, this is the first report that showed that the over-expression of Pti1-like gene enhances the salt resistance in plants.