Portable, Disposable, Paper-Based Microbial Fuel Cell Sensor Utilizing Freeze-Dried Bacteria for In Situ Water Quality Monitoring
Portable, Disposable, Paper-Based Microbial Fuel Cell Sensor Utilizing Freeze-Dried Bacteria for In Situ Water Quality Monitoring
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DOI：
10.1021/acsomega.0c01333
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发表时间：
2020-06-16
期刊：
影响因子：
4.1
通讯作者：
Choi, Seokheun
中科院分区：
文献类型：
--
作者：
Cho, Jong Hyun;Gao, Yang;Choi, Seokheun
随着人类活动的增加，水质监测正成为我们生活中必不可少的一部分，不断有未知和意想不到的污染物进入我们的水系统。为了确保向公众和生态系统提供安全和清洁的水，必须建立快速和敏感的水毒性监测现场预警系统。在这项工作中，一个完全基于纸的微生物燃料电池传感器利用冻干细菌被证明是一种便携式和一次性的水毒性传感器。将细菌细胞预先接种在设备的阳极储液器上，并将其冷冻干燥，使其现场和按需应用成为可能。在细菌与水样品再水化后，获得电流读数，并计算不同浓度的甲醛作为模型毒素的抑制率（IR）。对于0.001、0.01和0.02%的甲醛，IR分别为7.88、16.08和23.14%。这些IR与甲醛浓度在R-2 = 0.995处显示出非常好的线性。此外，对冻干微生物燃料电池传感器的保质期进行了研究。即使在4 ℃和-20 ℃的干燥器中储存14天后，与新器件相比，性能输出仍为96%。
Water quality monitoring is becoming an essential part of our lives as increasing human activities continue to spill unknown and unexpected contaminants into our water systems. To ensure the provision of safe and clean water to the public and the ecosystem, the development of rapid and sensitive in situ early warning systems for water toxicity monitoring is crucial. In this work, an entirely paper-based microbial fuel cell sensor utilizing freeze-dried bacteria is demonstrated as a portable and disposable water toxicity sensor. The bacterial cells were preinoculated on the anode reservoir of the device, and they were freeze-dried, making their on-site and on-demand applications possible. Upon rehydration of the bacteria with the water samples, current readings were obtained, and inhibition ratios (IRs) were calculated for different concentrations of formaldehyde as a model toxin. For 0.001, 0.01, and 0.02% of formaldehyde, IRs of 7.88, 16.08, and 23.14% were obtained, respectively. These IRs showed a very good linearity with the formaldehyde concentrations at R-2 = 0.995. Additionally, the shelf life of the freeze-dried microbial fuel cell sensor was investigated. Even after 14 days of storage in the desiccator, at 4, and at -20 degrees C, the performance outputs compared to the new device were all at 96%.