Proteomic analysis of therapeutic effects of Qingyi pellet on rodent severe acute pancreatitis-associated lung injury
Proteomic analysis of therapeutic effects of Qingyi pellet on rodent severe acute pancreatitis-associated lung injury
复制标题
清胰丸对啮齿动物重症急性胰腺炎相关性肺损伤治疗作用的蛋白质组学分析
DOI：
10.1016/j.biopha.2019.109300
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发表时间：
2019-10-01
影响因子：
7.5
通讯作者：
Chen, Hailong
中科院分区：
文献类型：
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作者：
Sun, Zhongwei;Li, Lei;Chen, Hailong
重症急性胰腺炎（severe acute pancreatitis，SAP）是临床常见的急腹症，以胰腺自身消化为特征。急性肺损伤是SAP的主要并发症之一，是SAP死亡率高的主要原因。中药清胰丸在临床上已广泛应用于SAP的治疗。本研究建立了重症急性胰腺炎相关肺损伤（SAP-ALI）大鼠模型，采用QYT治疗SAP-ALI大鼠，并采用质谱技术对QYT治疗后的SAP-ALI大鼠肺组织蛋白质组学进行分析。结果表明，SAP组有9种蛋白质发生了明显的变化。在9种蛋白质中，7种蛋白质（α 2-巨球蛋白、组织蛋白酶S、ras相关蛋白RAP-1A、整合素β、蛋白磷酸酶2A、细胞间粘附分子1和p38）表达上调，2种蛋白质（衔接分子Crk和stathmin）表达下调。有趣的是，QYT组的数据显示，衔接分子Crk和stathmin表达上调，而其他7种蛋白表达下调。京都基因百科全书显示，这些蛋白质作用于PI 3 K-AKT、趋化因子信号通路、细胞凋亡、白细胞跨内皮迁移和粘着斑。因此，QYT对SAP-ALI的治疗作用可能是通过许多组分的相加和/或协同相互作用实现的。
Severe acute pancreatitis (SAP) is a common acute clinical abdomen syndrome which is characterized by pancreatic self-digestion. As one of the major complication of SAP, acute lung injury is the main reason of high mortality. The traditional Chinese medicine Qingyi pellet (QYT) has been widely used for SAP in clinic. In our study, we constructed the severe acute pancreatitis-associated lung injury (SAP-ALI) rat model and treated with QYT, then characterized the protein from the lung tissue by using a mass spectrometry-based proteomic strategy. Our results showed that, in the SAP group, 9 proteins exhibited obvious changes according to the proteomic analysis. Among the 9 proteins, 7 proteins (alpha-2-macroglobulin, Cathepsin S, ras-related protein RAP-1A, integrin beta, protein phosphatase 2A, Intercellular adhesion molecule 1 and p38) were up-regulated, and 2 proteins (adapter molecule Crk and stathmin) were down-regulated. Interestingly, the data of the QYT group showed that adapter molecule Crk and stathmin were up-regulated, but the other 7 proteins were down-regulated. The kyoto encyclopedia of genes shows that the proteins act on PI3K-AKT, chemokine signaling pathways, apoptosis, leukocyte transendothelial migration and focal adhesion. Therefore, the therapeutic effects of QYT on SAP-ALI are potentially through the additive and/or synergistic interactions of numerous components.