Differential effects of chronic monocyte depletion on macrophage populations.
Differential effects of chronic monocyte depletion on macrophage populations.
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发表时间：
1983-09
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通讯作者：
A. Volkman;N. Chang;P. Strausbauch;P. Morahan
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作者：
A. Volkman;N. Chang;P. Strausbauch;P. Morahan
向小鼠施用趋骨同位素89 Sr导致严重的单核细胞减少症，但对驻留的腹膜巨噬细胞（Mphi）的数量没有任何明显影响。通过确定89 Sr治疗后残留的血液单核细胞是否仍然是Mphi的有效来源来解释这种二分法。干细胞计数表明，90%的骨髓巨噬细胞集落形成细胞后，89锶伴随着10倍的脾M-CFC上升。然而，脾切除术前进行89锶治疗，导致一点额外的单核细胞减少症，并没有影响居民腹膜Mphi的数量，即使采样延长到31天，间隔超过了腹膜Mphi的可接受的半衰期。腹腔注射巯基乙酸盐或短棒状杆菌引起很少或没有单核细胞-Mphi在各自的间隔4和7天。在89 Sr后26天，在氚化胸腺嘧啶标记的小鼠中尝试用巯基乙酸盐激发。四天后，只有2倍增加标记腹膜Mphi被发现在89锶治疗的小鼠相比，对照组增加150倍。对胞外酶5 '-核苷酸酶、碱性磷酸二酯酶I和亮氨酸氨肽酶在这种诱导实验中的研究表明，观察到的活性变化反映了常驻Mphi的直接刺激，而不是单核细胞迁移。总体而言，结果表明，89 Sr治疗区分两个大的Mphi群体的基础上，他们对骨髓的依赖。炎症的Mphi反映了单核细胞减少症，并且通过这种治疗严重且快速地消耗。另一方面，Mphi常驻型的维持似乎与骨髓的状态和血液中单核细胞的水平无关。
The administration of the bone-seeking isotope, 89Sr, to mice results in severe monocytopenia without any apparent effect on the numbers of resident peritoneal macrophages (Mphi). An explanation for this dichotomy was sought by determining whether the residual blood monocytes were still an effective source of Mphi after 89Sr treatment. Stem cell enumeration showed that a 90% fall in bone marrow macrophage colony-forming cells after 89Sr was accompanied by a 10-fold rise in splenic M-CFC. Splenectomy performed before 89Sr treatment, however, resulted in little additional monocytopenia and had no affect on the numbers of resident peritoneal Mphi even when sampling was extended to 31 days, an interval beyond the accepted half-time for peritoneal Mphi. Intraperitoneal injections of thioglycollate or Corynebacterium parvum elicited few or no monocyte-Mphi during respective intervals of 4 and 7 days. Elicitation with thioglycollate was attempted in tritiated thymidine-labeled mice 26 days after 89Sr. Four days later only a 2-fold increase in labeled peritoneal Mphi was found in the 89Sr-treated mice compared with a 150-fold increase in the controls. Studies of the ectoenzymes 5'-nucleotidase, alkaline phosphodiesterase I, and leucine aminopeptidase in such elicitation experiments suggested that the observed changes in activities reflected the direct stimulation of resident Mphi rather than monocyte immigration. Overall, the results indicate that treatment with 89Sr distinguishes two large populations of Mphi on the basis of their dependence on bone marrow. Mphi of inflammation reflect the monocytopenia and are severely and rapidly depleted by such treatment. The maintenance of resident type Mphi, on the other hand, appears to be independent of both the state of the bone marrow and the level of monocytes in the blood.