Activity of single ion channel proteins detected with a planar microstructure
Activity of single ion channel proteins detected with a planar microstructure
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DOI：
10.1063/1.1531228
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发表时间：
2002-12-16
影响因子：
4
通讯作者：
Behrends, JC
中科院分区：
文献类型：
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作者：
Fertig, N;Klau, M;Behrends, JC
我们展示了使用平面微结构玻璃芯片获得的单离子通道蛋白介导的电流记录。在这些芯片中，直径为1-2μm的孔是通过离子径迹蚀刻产生的，并用于膜片钳，而不是使用传统的微量移液器。我们的结果代表了使用此类设备从细胞膜的单个通道进行记录的成功。平面芯片极大地增强了含有膜的离子通道的可及性，并且可以作为使用膜片钳电流记录与其他单分子技术的组合进行单离子通道实验的工作台。 (C) 2002 年美国物理研究所。
We present recordings of currents mediated by single ion channel proteins obtained using planar, microstructured glass chips. In these chips, pores with diameters of 1-2 mum are produced by ion track etching and are used for patch clamping instead of using the classical micropipette. Our results represent success in using such devices to record from single channels in cell membranes. The planar chip greatly enhances the accessibility of the ion channel containing membrane and can serve as a workbench for experiments on single ion channels using combinations of patch clamp current recording with other single molecule techniques. (C) 2002 American Institute of Physics.