Many of the IgA producing plasma cells in murine gut are derived from self-replenishing precursors in the peritoneal cavity
Many of the IgA producing plasma cells in murine gut are derived from self-replenishing precursors in the peritoneal cavity
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DOI：
10.1093/intimm/1.1.75
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发表时间：
1989-01-01
影响因子：
4.4
通讯作者：
Herzenberg, Leonore A.
中科院分区：
文献类型：
--
作者：
Kroese, Frans G. M.;Butcher, Eugene C.;Herzenberg, Leonore A.
本文使用长期B谱系嵌合体来研究小鼠中浆细胞的起源。嵌合小鼠通过用来自Igh-C同种异型不同的同类对小鼠的腹膜细胞（PerC）和骨髓细胞重建致死性照射的小鼠来构建。这些小鼠中的所有常规B细胞表达骨髓供体的同种异型，并且几乎所有Ly-1 B谱系细胞表达PerC供体的同种异型。免疫组织化学和PACS分析表明，这些螨的外周淋巴器官中几乎所有的（sIg+）B细胞均来源于骨髓供者。然而，尽管在这些动物中骨髓来源的B细胞数量巨大，但淋巴器官和肠道的免疫组织学染色显示，几乎所有的IgM、IgG和伊加浆细胞都来源于PerC供体。这些数据表明，腹膜腔含有自我补充细胞的主要储库，其在粘膜免疫应答中起重要作用。讨论了这些是属于Ly-1 B谱系的B细胞的可能性。
Long term B lineage chimeras are used here to study the origin of plasma cells in the mouse. Chimeric mice are constructed by reconstituting lethally irradiated mice with peritoneal cells (PerC) and bone marrow cells from congenic pairs of mice differing In Igh-C allotype. All conventional B cells in these mice express the allotype of the bone marrow donor and nearly all Ly-1 B lineage cells express the allotype of the PerC donor. PACS analysis and immunohistology of these mite shows that virtually all (slg+) B cells In peripheral lymphoid organs are derived from the bone morrow donor. However, despite this overwhelming number of bone marrow-derived B cells in those animals, immunohistological staining of lymphoid organs and gut shows that nearly hail of the IgM, IgG, and IgA plasma cells derive from the PerC donor. These data demonstrate that the peritoneal cavity contains a major reservoir of self-replenishing cells that play a significant role in the mucosal immune response. The possibility that these are B cells that belong to the Ly-1 B lineage is discussed.