Effects of Polyamines (Putrescine, Spermidine and Spermine) on root tip mitosis and chromosomes in Allium cepa L.
Effects of Polyamines (Putrescine, Spermidine and Spermine) on root tip mitosis and chromosomes in Allium cepa L.
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DOI：
10.1508/cytologia.70.217
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发表时间：
2005-06-01
期刊：
影响因子：
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通讯作者：
Bozcuk, Suna
中科院分区：
文献类型：
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作者：
Gomurgen, Ayse Nihal;Mutlu, Fatma;Bozcuk, Suna
本文研究了腐胺(Put)、亚精胺(Spd)和精胺(Spm)对洋葱根尖有丝分裂的影响。用0.01、1和2 mM浓度的多胺(PA)处理洋葱根尖3、6、12、24和48h，用Feulgen染色的永久根尖南瓜标本进行观察。这项研究证实了Pas对染色体有多种影响。PA浓度对有丝分裂指数(MI)有明显的抑制作用，但在处理6h时，SPM、Spd和Put在所有浓度下均有增加MI的作用。与对照相比，多胺影响每个有丝分裂阶段的相对持续时间。诱发的异常类型有：染色体粘连、c-中期、后期和末期桥、后期和末期染色体异常、后期和末期染色体异常、后期和末期染色体标记和前移。间期细胞可见双核细胞和微核形成。
In the present study effects of the polyamines; putrescine (Put), spermidine (Spd) and spermine (Spm) have been studied on root tip mitosis of Allium cepa L. Root tips of A. cepa were treated with a series of polyamine (PA) concentrations, ranging from 0.01, 1 and 2 mM for 3, 6, 12, 24 and 48 h. The roots were examined in permanent root tip squash preparations stained by the Feulgen technique. This research has confirmed that PAs have various effects on chromosomes. PA concentrations used, generally had a marked mitodepressive effect on mitotic index (MI) but at 6 h treatment of Spm, Spd and Put at all concentrations had increased MI. Polyamines affected the relative duration of each mitotic stage as compared to the control. They also increased frequency of abnormal mitosis.The types of abnormalities induced are: chromosome stickiness, c-metaphase, anaphase and telophase bridges, disturbed chromosomes of anaphase and telophase stages, tagging and forward chromosomes at anaphase and telophase. Binucleate cells and micronuclei formation at interphase cells were also observed.