ASSOCIATIONS OF METABOLIC PARAMETERS AND ETHANOL CONSUMPTION WITH MESSENGER RNA EXPRESSION OF CLOCK GENES IN HEALTHY MEN
ASSOCIATIONS OF METABOLIC PARAMETERS AND ETHANOL CONSUMPTION WITH MESSENGER RNA EXPRESSION OF CLOCK GENES IN HEALTHY MEN
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DOI：
10.3109/07420520903398617
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发表时间：
2010-01-01
影响因子：
2.8
通讯作者：
Fujimura, Akio
中科院分区：
文献类型：
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作者：
Ando, Hitoshi;Ushijima, Kentarou;Fujimura, Akio
最近的研究表明，生物钟功能的受损会导致各种病理情况，如肥胖、糖尿病和酒精中毒，在这些情况下发现时钟基因的mRNA表达发生了变化。然而，即使在健康人中，时钟基因的表达是否会根据代谢条件的不同而变化仍有待确定。为了解决这个问题，我们研究了代谢参数和酒精摄入量与时钟基因(CLOCK，BMAL1，PER1，PER2和PER3)mRNA表达的关系。29名健康的非肥胖老年男性(年龄51-78岁)，通过在接近09：00小时的一次静脉采血，研究了代谢参数和饮酒与时钟基因(CLOCK，BMAL1，PER1，PER2和PER3)mRNA表达的关系。(1)腰围与PER1基因水平(r=-0.43)，(2)血糖浓度与PER2(r=-0.50)，(3)酒精摄入量与BMAL1(r=-0.43)；(4)血清γ-GTP浓度(酒精摄入量的敏感指标)和PER2(r=-0.40)。这些结果表明，时钟基因的mRNA表达与肥胖、葡萄糖耐量和酒精消耗有关，甚至在健康人中也是如此。(作者通讯：akiofuji@jichi.ac.jp)
Recent studies suggest that the impairment of circadian clock function causes various pathological conditions, such as obesity, diabetes, and alcoholism, and an altered mRNA expression of clock genes was found under these conditions. However, it remains to be determined whether clock gene expression varies depending on metabolic conditions even in healthy people. To address this issue, we investigated the associations of metabolic parameters and alcohol consumption with mRNA expression of clock genes (CLOCK, BMAL1, PER1, PER2, and PER3) in peripheral blood cells obtained from 29 healthy non-obese elderly men (age 51-78 yrs) who adhered to a regular sleep-wake routine, through a single time-of-day venous blood sampling at similar to 09:00 h. There were significant correlations between (1) waist circumference and mRNA level of PER1 (r = -0.43), (2) plasma glucose concentration and PER2 (r = -0.50), (3) ethanol consumption and BMAL1 (r = -0.43), and (4) serum gamma-GTP concentration (a sensitive marker of alcohol consumption) and PER2 (r = -0.40). These results suggest mRNA expression of clock genes is associated with obesity, glucose tolerance, and ethanol consumption even in healthy people. (Author correspondence: akiofuji@jichi.ac.jp)