SEPARATION OF D-AMINO AND L-AMINO-ACIDS BY LIQUID-CHROMATOGRAPHY - USE OF CHIRAL ELUANTS
SEPARATION OF D-AMINO AND L-AMINO-ACIDS BY LIQUID-CHROMATOGRAPHY - USE OF CHIRAL ELUANTS
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DOI：
10.1126/science.36662
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发表时间：
1979-01-01
期刊：
影响因子：
56.9
通讯作者：
GILAV, E
中科院分区：
文献类型：
--
作者：
HARE, PE;GILAV, E
含有手性铜-脯氨酸复合物的水性洗脱液可在离子交换柱上分离未衍生化的氨基酸对映体。立体选择性是由于溶液中形成的非对映氨基酸-Cu络合物的稳定性差异造成的。洗脱液手性的简单改变就会颠倒对映体洗脱的顺序。为了检测和定量皮摩尔量的氨基酸，在与邻苯二甲醛反应后监测洗脱液的荧光，邻苯二甲醛是一种对脯氨酸不敏感但对含有伯氨基的氨基酸高度敏感的试剂。
An aqueous eluant containing a chiral Cu-proline complex causes the separation of underivatized amino acid enantiomers on an ion-exchange column. The stereoselectivity is due to differences in stability of the diastereomeric amino acid-Cu complexes formed in solution. A simple change in the chirality of the eluant reverses the order of the enantiomer elution. For detection and quantification of picomole amounts of amino acids, the eluant is monitored for fluorescence after reaction with o-phthalaldehyde, a reagent insensitive to proline but highly sensitive for amino acids containing a primary amino group.