Molecular mapping of resistance to blight in an interspecific cross in the genus Castanea
Molecular mapping of resistance to blight in an interspecific cross in the genus Castanea
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DOI：
10.1094/phyto.1997.87.7.751
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发表时间：
1997-07-01
期刊：
影响因子：
3.2
通讯作者：
Doudrick, RL
中科院分区：
文献类型：
--
作者：
Kubisiak, TL;Hebard, FV;Doudrick, RL
以美国板栗×中国板栗3代家系为材料，构建了板栗遗传连锁图谱，并研究了板栗对栗疫病菌（Endothia parasitica）的抗性控制。对一个由102个个体组成的F-2家系进行了241个多态性标记（8个同工酶、17个限制性片段长度多态性（RFLPs）和216个随机扩增多态性DNA（RAPDs））的DNA基因型分析。在这些标记中，196个如预期分离，随后用于初级连锁作图。两种同工酶，12个RFLPs，和170个RAPD被映射到12个连锁群，跨越总遗传距离为530.1 Kosambi厘摩。评价F-2植株对E.寄生虫感染的直接接种他们与两个独特的真菌分离物和测量溃疡扩展超过3.5个月的时间。将结果与标记基因型数据进行比较，从而鉴定与抗性反应显著相关的基因组区域。单标记或非同时方差分析确定了7个基因组区域，似乎有主机响应的影响。多标记或同步模型表明，这些地区有三个主机响应的显着影响，一起解释高达42.2%的溃疡病大小的总变化。在每一个假定的抗性位点，来自板栗祖父母的等位基因与较小的溃疡大小，或较高水平的电阻。
A three-generation American chestnut x Chinese chestnut pedigree was used to construct a genetic linkage map for chestnut and to investigate the control of resistance to Endothia parasitica (chestnut blight fungus). DNA genotypes for 241 polymorphic markers (eight isozymes, 17 restriction fragment length polymorphisms [RFLPs], and 216 random amplified polymorphic DNAs [RAPDs]) were assayed on an F-2 family consisting of 102 individuals. Of these markers, 196 were segregating as expected and, subsequently, used for primary linkage mapping. Two isozymes, 12 RFLPs, and 170 RAPDs were mapped to 12 linkage groups spanning a total genetic distance of 530.1 Kosambi centimorgans. F-2 plants were evaluated for a response to E. parasitica infection by directly inoculating them with two unique fungal isolates and measuring canker expansion over a period of 3.5 months. Results were compared with the marker genotype data, thereby identifying genomic regions significantly associated with a resistance response. Single-marker or nonsimultaneous analyses of variance identified seven genomic regions that appear to have an effect on host response. Multiple-marker or simultaneous models suggest that three of these regions have a significant effect on host response, together explaining as much as 42.2% of the total variation for canker size. At each of the three putative resistance loci, alleles derived from the Chinese chestnut grandparent were associated with smaller canker size, or higher levels of resistance.