The expression of two DEFICIENS-like genes was reduced in the sepaloid tepals of viridiflora tulips
The expression of two DEFICIENS-like genes was reduced in the sepaloid tepals of viridiflora tulips
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DOI：
10.1270/jsbbs.60.110
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发表时间：
2010-06-01
期刊：
影响因子：
2.4
通讯作者：
Kanno, Akira
中科院分区：
文献类型：
--
作者：
Hirai, Masayo;Ochiai, Toshinori;Kanno, Akira
根据改进的ABC模型，B类基因在轮生1和轮生2、3中表达，因此在许多单子叶植物中，轮生1和2的器官具有相同的瓣状结构。郁金香是著名的郁金香品种群，其花序I和2中的花被片有绿色条纹。根据改进的ABC模型，该表型可能为B类突变体。在这项研究中，我们发现花被表型与雄蕊表型相关。我们从野生型郁金香中分离到两个A类基因(TGSQA和TGSQB)。这两个A类基因与B类基因(TGDEFA、TGDEFB和TGGLO)的Northern杂交表明，TGDEFA和TGDEFB在郁金香中的表达弱于野生型郁金香，而其余基因的表达模式几乎相同。根据我们的结果，我们认为两个DEF类基因TGDEFA和TGDEFB的表达减少参与了病毒菌表型的发育。这种表达的降低可能是由于氨基酸的差异造成的。
According to the modified ABC model, class B genes are expressed in whorl 1 as well as in whorls 2 and 3, therefore the organs of whorls 1 and 2 have the same petaloid structure in many monocots. The viridiflora tulip, a well-known cultivar group, has flowers in which the tepals in whorls I and 2 have greenish stripes. This phenotype could be expected as class B mutant by the modified ABC model. In this study, we discovered that tepal phenotypes correlated with that of stamens. We isolated two class A genes (TGSQA and TGSQB) from wild type tulip. Northern hybridization of these two class A genes, and the class B genes (TGDEFA, TGDEFB and TGGLO), showed that the expression of TGDEFA and TGDEFB in the viridiflora tulips were weaker than those in wild type cultivars, whereas the expression patterns of the rest genes were almost identical. From our results, we suggest that reduced expression of the two DEF-like genes, TGDEFA and TGDEFB, is involved in the development of the viridiflora phenotype. This reduced expression could be caused by the amino acid difference.