Are laboratories following published recommendations for lupus anticoagulant testing?
Are laboratories following published recommendations for lupus anticoagulant testing?
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DOI：
10.1160/th08-06-0353
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发表时间：
2009-01-01
影响因子：
6.7
通讯作者：
Hayward, Catherine R.
中科院分区：
文献类型：
--
作者：
Moffat, Karen A.;Ledford-Kraemer, Marlies R.;Hayward, Catherine R.
狼疮抗凝剂（LA）的实验室检查通常用于评估血栓形成或疑似磷脂抗体综合征。为了确定当前的LA检测实践，以及它们是否符合已发表的建议，向北美专业凝血实验室协会（NASCOLA）和ECAT基金会（ECAT）的临床实验室成员分发了两份问卷。第一份和第二份问卷分别由113家和96家实验室完成。常用的LA试验包括稀释的拉塞尔蝰蛇毒时间、LA敏感的活化部分凝血活酶时间和六角磷脂试验。尽管一些实验室在要求时进行了单次LA试验，但大多数实验室遵守了已发表的建议：使用贫血小板血浆进行LA试验;使用两种或更多种筛选试验（代表不同的测定原理）和一种具有低磷脂浓度的测定法排除LA;通过提供异常LA筛选的方法确认LA磷脂依赖性;记录合并正常血浆中的抑制剂活性;而不是用磷脂抗体来确认LA少数民族（
Laboratory tests for lupus anticoagulants (LA) are commonly performed to evaluate thrombosis or suspected phospholipid antibody syndromes. To determine current LA testing practices, and if they conform to published recommendations, two questionnaires were distributed to clinical laboratory members of the North American Specialized Coagulation Laboratory Association (NASCOLA) and the ECAT Foundation (ECAT). The first and second questionnaires were completed by 113 and 96 laboratories, respectively. Commonly performed LA tests included the dilute Russell's viper venom time, LA sensitive activated partial thromboplastin time and hexagonal phospholipid test. Although some laboratories did single LA tests if requested, the majority complied with published recommendations: to use platelet poor plasma for LA tests; to use two or more screening tests, representing different assay principles,and one assay having a low phospholipid concentration to exclude LA; to confirm LA phospholipid dependency by the method giving an abnormal LA screen;to document the inhibitor activity on pooled normal plasma; and not to use phospholipid antibodies to confirm LA. A minority (