A simple and sensitive fluorometric assay for tyrosine hydroxylase.
A simple and sensitive fluorometric assay for tyrosine hydroxylase.
复制标题
一种简单而灵敏的酪氨酸羟化酶荧光测定法。
DOI：
10.1016/0003-2697(78)90735-2
复制
发表时间：
1978
影响因子：
2.9
通讯作者：
H. Fujisawa
中科院分区：
文献类型：
--
作者：
T. Yamauchi;H. Fujisawa
本文介绍了一种简便、快速、灵敏的酪氨酸羟化酶活性荧光测定法。通过将酪氨酸转化为3，4-二羟基苯丙氨酸（多巴），然后通过三羟基吲哚法将其转化为高荧光产物来检测酶活性。该测定方法是非常可重复的，更敏感的放射化学方法的酪氨酸羟化酶活性的测定使用的[3 H]水的分离常用的，线性从0.2至12 nmol的多巴。该方法适用于活性范围较广的酶的测定。
A simple, rapid, and sensitive fluorometric assay for measuring the activity of tyrosine hydroxylase is described. The enzyme activity is detected by converting tyrosine to 3,4-dihydroxyphenylalanine (dopa), which is then subjected to conversion to the highly fluorescent product by the trihydroxyindole method. The assay method is very reproducible, more sensitive than a radiochemical method for the determination of tyrosine hydroxylase activity using the isolation of [3H]water commonly used, and linear from 0.2 to 12 nmol of dopa. The method should be applicable for the assay of the enzyme with a wide range of activity.