Types of bipolar cells in the mouse retina
Types of bipolar cells in the mouse retina
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DOI：
10.1002/cne.10985
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发表时间：
2004-01-26
影响因子：
2.5
通讯作者：
Wässle, H
中科院分区：
文献类型：
--
作者：
Ghosh, KK;Bujan, S;Wässle, H
我们通过注射荧光黄和神经生物素研究了小鼠视网膜固定垂直组织切片（切片）中双极细胞的形态。9个不同的锥双极细胞类型和一个杆双极细胞类型的区别。分类的主要标准是其轴突终末在内丛状层（IPL）的分支模式和分层水平。为了评估这一点，IPL被细分为五个等宽的层。对切片进行钙视网膜蛋白免疫染色，钙视网膜蛋白标记三条水平带，作为轴突末端精确定位的标准测量。用G蛋白Ggamma 13（一种ON双极细胞的标记物）的抗体对视网膜进行免疫染色，使得分离OFF双极细胞和ON双极细胞成为可能。用抗神经激肽3受体（NK 3R）的抗体对至少两个OFF-视锥双极细胞（1型和2型）进行免疫标记。另一个OFF-和ON-锥双极细胞（3型和5型）用抗钙结合蛋白CaB 5的抗体进行免疫染色。这里描述的双极细胞类型进行了比较与以前的计划，大鼠和灵长类动物的双极细胞。讨论了这三个种之间的同源类型。
We studied the morphology of bipolar cells in fixed vertical tissue sections (slices) of the mouse retina by injecting the cells with Lucifer Yellow and Neurobiotin. Nine different cone bipolar cell types and one rod bipolar cell type were distinguished. The major criteria for classifying the cells were the branching pattern and stratification level of their axon terminals in the inner plexiform layer (IPL). To assess this, the IPL was subdivided into five strata of equal width. The slices were immunostained for calretinin, which labels three horizontal bands serving as a standard measure for the precise localization of the axon terminals. Immunostaining the retina with antibodies against the G-protein Ggamma13, a marker for ON-bipolar cells, made it possible to separate OFF- and ON-bipolar cells. At least two OFF-cone bipolar cells (Types 1 and 2) were immunolabeled with antibodies against the neurokinin 3 receptors (NK3R). A further OFF- and an ON-cone bipolar cell (Types 3 and 5) were immunostained with antibodies against the calcium-binding protein CaB5. The bipolar cell types described here were compared with previous schemes of rat and primate bipolar cells. Homologous types between the three species are discussed.