Pseudidiomarina donghaiensis sp. nov. and Pseudidiomarina maritima sp. nov., isolated from the East China Sea.
Pseudidiomarina donghaiensis sp. nov. and Pseudidiomarina maritima sp. nov., isolated from the East China Sea.
复制标题
DOI：
10.1099/ijs.0.005702-0
复制
发表时间：
2009-06
影响因子：
2.8
通讯作者：
Yue-hong Wu;Yuanyuan Shen;Xue-Wei Xu;Chun-sheng Wang;A. Oren;Min Wu
中科院分区：
文献类型：
--
作者：
Yue-hong Wu;Yuanyuan Shen;Xue-Wei Xu;Chun-sheng Wang;A. Oren;Min Wu
从东海采集的海水样品中分离出两种革兰氏阴性、需氧、能运动的杆状细菌，命名为菌株 908033(T) 和 908087(T)。两种分离株的化学分类学特征包括存在 iso-C(15:0)、iso-C(17:0) 和 iso-C(17:1)omega9c 作为主要细胞脂肪酸，以及 Q-8 作为主要泛醌。菌株908033(T)和908087(T)的基因组DNA G+C含量分别为45.5和45.2mol%。基于16S rRNA基因序列的系统发育分析表明，新分离株与假海藻属成员相关，与该属已识别物种的模式株相似度为95.8-96.6%。这两个分离株与Pseudidiomarina sediminum CICC 10319(T)的DNA-DNA杂交实验结果，结合化学分类学和表型数据，表明新分离株代表了Pseudidiomarina属的两个新种，其名称为Pseudidiomarina donghaiensis sp.。十一月（模式菌株 908033(T)=CGMCC 1.7284(T)=JCM 15533(T)）和 Pseudidiomarina maritima sp.十一月(型菌株908087(T)=CGMCC 1.7285(T)=JCM 15534(T))被提出。
Two Gram-negative, aerobic, motile, rod-shaped bacteria, designated strains 908033(T) and 908087(T), were isolated from a seawater sample collected from the East China Sea. Chemotaxonomic characteristics of the two isolates included the presence of iso-C(15 : 0), iso-C(17 : 0) and iso-C(17 : 1)omega9c as the major cellular fatty acids and Q-8 as the predominant ubiquinone. The genomic DNA G+C contents of strains 908033(T) and 908087(T) were 45.5 and 45.2 mol%, respectively. Phylogenetic analyses based on 16S rRNA gene sequences revealed that the new isolates were related to members of the genus Pseudidiomarina, showing levels of similarity of 95.8-96.6 % with the type strains of recognized species of the genus. The results of DNA-DNA hybridization experiments among these two isolates and Pseudidiomarina sediminum CICC 10319(T), in combination with chemotaxonomic and phenotypic data, demonstrated that the new isolates represent two novel species of the genus Pseudidiomarina, for which the names Pseudidiomarina donghaiensis sp. nov. (type strain 908033(T)=CGMCC 1.7284(T)=JCM 15533(T)) and Pseudidiomarina maritima sp. nov. (type strain 908087(T)=CGMCC 1.7285(T)=JCM 15534(T)) are proposed.