DNA-templated synthesis of biomimetic cell wall for nanoencapsulation and protection of mammalian cells
DNA-templated synthesis of biomimetic cell wall for nanoencapsulation and protection of mammalian cells
复制标题
DOI：
10.1038/s41467-019-10231-y
复制
发表时间：
2019-05
影响因子：
16.6
通讯作者：
Peng Shi;Nan Zhao;James Coyne;Yong Wang
中科院分区：
文献类型：
--
作者：
Peng Shi;Nan Zhao;James Coyne;Yong Wang
哺乳动物细胞与植物和微生物细胞不同，没有外部细胞壁保护。环境攻击很容易损害或破坏哺乳动物细胞。因此，在其质膜上开发仿生细胞壁（BCW）作为屏障的能力可以推进各种应用。在这里，我们展示了BCW的合成与框架模板和交联矩阵屏蔽活的哺乳动物细胞。框架模板是一种超分子DNA结构。交联基质是由藻酸盐和聚赖氨酸制成的交联复合物。由于BCW合成的整个过程严格在生理条件下操作，因此BCW覆盖的哺乳动物细胞可以保持高的生物活性。更重要的是，数据显示BCW不仅可以保护活的哺乳动物细胞免受物理攻击，还可以保护它们免受生物攻击。因此，这项研究已经成功地证明了BCW在活的哺乳动物细胞上的合成具有保护它们免受环境攻击的巨大潜力。
Mammalian cells are different from plant and microbial cells, having no exterior cell walls for protection. Environmental assaults can easily damage or destroy mammalian cells. Thus, the ability to develop a biomimetic cell wall (BCW) on their plasma membrane as a shield can advance various applications. Here we demonstrate the synthesis of BCW with a framing template and a crosslinked matrix for shielding live mammalian cells. The framing template is a supramolecular DNA structure. The crosslinked matrix is a polyelectrolyte complex made of alginate and polylysine. As the entire procedure of BCW synthesis is strictly operated under physiological conditions, BCW-covered mammalian cells can maintain high bioactivity. More importantly, the data show that BCW can shield live mammalian cells from not only physical assaults but also biological assaults. Thus, this study has successfully demonstrated the synthesis of BCW on live mammalian cells with great potential of shielding them from environmental assaults.