Angiogenesis and improved cerebral blood flow in the ischemic boundary area were detected after electroacupuncture treatment to rats with ischemic stroke
Angiogenesis and improved cerebral blood flow in the ischemic boundary area were detected after electroacupuncture treatment to rats with ischemic stroke
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DOI：
10.1179/016164110x12714125204317
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发表时间：
2011-01-01
影响因子：
1.9
通讯作者：
Fan, Xiaonong
中科院分区：
文献类型：
--
作者：
Du, Yuanhao;Shi, Lei;Fan, Xiaonong
目的：针刺对脑缺血有效，促进血管生成被认为是缺血性脑卒中的潜在治疗方法。最近的数据也表明，血管生成确实对大脑循环和行为改善有好处。我们观察了电针对缺血性脑卒中模型血管内皮细胞增殖的影响。方法：采用大脑中动脉闭塞法（MCAO）诱导大鼠局部缺血。将大鼠随机分为两组：对照组（MCAO）和电针组（MCAO +电针）。采用双免疫荧光标记法（Ki67和vWF）染色血管内皮细胞（EC）增殖，采用激光多普勒血流仪（LDF）测定脑区血流量（rCBF），并进行神经学评分。在闭锁期用电针刺激GV26（水沟）穴。结果：对照组细胞增殖从24 h开始，持续至少7 d， 3 d达到最大诱导，7 d后细胞增殖减少，12 d无细胞增殖。而电针组在MCAO后12 h开始细胞增殖，且在1、2、3、7 d细胞数量较对照组显著增加(P
Objectives: Acupuncture has been known to be effective in cerebral ischemia, and promoting angiogenesis is considered as a potential therapy of ischemic stroke. Recent data have also shown that angiogenesis indeed brings benefit to cerebral circulation and behavior improvement. We examined the effect of electroacupuncture on vascular endothelial cell proliferation in the ischemic stroke model.Methods: A local ischemia was induced using the method of middle cerebral artery occlusion (MCAO) in rat. Rats were randomly divided into two groups: the control group (with MCAO) and the electroacupuncture group (with MCAO + electroacupuncture). The vascular endothelial cell (EC) proliferation were stained by double-immunofluorescence labeling method (Ki67 and vWF), region cerebral blood flow (rCBF) was measured using laser Doppler flowmeter (LDF), and the neurological scores was assessed. Electroacupuncture stimulation was applied to the acupoint GV26 (Shuigou) during the occlusion period.Results: In the control group, the EC proliferation started at 24 hours, was sustained for at least 7 days with a maximum induction at 3 days, decreased thereafter at 7 days, and was absent at 12 days after MCAO. However, EC proliferation started at 12 hours after MCAO in electroacupuncture group, and increased significantly in the number of cells at 1, 2, 3, and 7 days compared with control group (P