Gene expression profiles of vitrified in vitro- and in vivo-derived bovine blastocysts
Gene expression profiles of vitrified in vitro- and in vivo-derived bovine blastocysts
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DOI：
10.1002/mrd.22068
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发表时间：
2012-09-01
影响因子：
2.5
通讯作者：
Agca, Yuksel
中科院分区：
文献类型：
--
作者：
Aksu, Digdem Aktoprakligil;Agca, Cansu;Agca, Yuksel
玻璃化已成为胚胎着床前冷冻保存的首选方法。本研究的目的是确定体内和体外生产的牛胚胎玻璃化后的差异表达基因。体外（IVF）和体内来源（IVV）的牛囊胚鉴定如下：体外产生的新鲜囊胚（IVF- f），体外产生的玻璃化囊胚（IVF- v），体内来源的新鲜囊胚（IVF- f），体内来源的玻璃化囊胚（IVV- v）。微阵列结果显示，53个基因在IVF和IVV之间存在差异调控，121个基因在新鲜囊胚和玻璃化囊胚之间存在差异调控(P？
Vitrification is becoming a preferred method for pre-implantation embryo cryopreservation. The objective of this study was to determine the differentially expressed genes of in vivo- and in vitro-produced bovine embryos after vitrification. In vitro- (IVF) and in vivo-derived (IVV) bovine blastocysts were identified as follows: in vitro-produced fresh (IVF-F), in vitro-produced vitrified (IVF-V), in vivo-derived fresh (IVV-F), in vivo-derived vitrified (IVV-V). The microarray results showed that 53 genes were differentially regulated between IVF and IVV, and 121 genes were differentially regulated between fresh and vitrified blastocysts (P?