Growth-stimulatory action of plasma membrane-associated growth factor. A synergistic effect with platelet-poor plasma.
Growth-stimulatory action of plasma membrane-associated growth factor. A synergistic effect with platelet-poor plasma.
复制标题
质膜相关生长因子的生长刺激作用。
DOI：
10.1016/0014-4827(87)90126-1
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发表时间：
1987
影响因子：
3.7
通讯作者：
Lieberman,MA
中科院分区：
文献类型：
--
作者：
Nagasaki,T;Lieberman,MA
生长因子活性部分纯化小鼠肝质膜和培养的小鼠成纤维细胞的生长刺激作用进行了研究。当作为生长因子的唯一来源时，质膜相关生长因子（PMGF）不能支持单层小鼠成纤维细胞的增殖。然而，在CM-Sephadex处理的人贫血小板血浆（h-CMP）存在下，它刺激成纤维细胞的生长，而h-CMP本身并不刺激生长。在静止的成纤维细胞中的DNA合成的刺激也观察到后，添加PMGF和h-CMP。在相同条件下，血小板衍生生长因子（PDGF）和成纤维细胞生长因子（FGF）显示出与PMGF相同的效果。h-CMP与PMGF对静止细胞的协同作用部分地由微克量的胰岛素或纳克量的胰岛素样生长因子I（IGF-I）再现。因此，本文提供的数据表明，PMGF的作用类似于称为“感受态因子”的生长因子家族，而不同于称为“进展因子”的血浆生长因子。
Growth factor activity was partially purified from mouse liver plasma membranes and its growth-stimulatory action on cultured mouse fibroblasts was studied. The plasma membrane-associated growth factor (PMGF) was unable to support the proliferation of mouse fibroblasts in monolayer when added as the sole source of growth factor. However, it stimulated the growth of fibroblasts in the presence of CM-Sephadex-treated human platelet-poor plasma (h-CMP) which by itself is not growth-stimulatory. The stimulation of DNA synthesis in quiescent fibroblasts was also observed upon the addition of PMGF and h-CMP. Under the same conditions, both platelet-derived growth factor (PDGF) and fibroblast growth factor (FGF) showed the same effect as did PMGF. The synergistic action of h-CMP with PMGF on quiescent cells was partially reproduced by insulin at microgram quantities or by insulin-like growth factor I(IGF-I) at nanogram quantities. Thus, the data presented here indicates that the action of PMGF is similar to that of the family of growth factors termed ‘competence factor’, and distinct from that of plasma growth factors termed ‘progression factor’.