Nanotentacle-Structured Magnetic Particles for Efficient Capture of Circulating Tumor Cells
Nanotentacle-Structured Magnetic Particles for Efficient Capture of Circulating Tumor Cells
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DOI：
10.1002/smll.201402619
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发表时间：
2015-04-24
期刊：
影响因子：
13.3
通讯作者：
Kim, Hak-Sung
中科院分区：
文献类型：
--
作者：
Jo, Seong-Min;Lee, Joong-Jae;Kim, Hak-Sung
循环肿瘤细胞（CTC）作为诊断和监测癌症状态的有前景的标记物引起了相当大的关注。尽管在分离 CTC 方面取得了许多技术进步，但捕获效率和纯度仍然是限制临床实践的挑战。在这里，展示了使用 M13 噬菌体构建“纳米触手”结构的磁性颗粒及其在有效捕获 CTC 方面的应用。 M13-噬菌体与磁性颗粒结合，然后用 PEG 修饰，并进一步束缚抗表皮受体 2 (HER2) 的单克隆抗体。即使对于全血中较少数量的癌细胞（大约 25 个细胞），使用纳米触手结构的磁性颗粒也能实现高捕获纯度（>45%）和效率（>90%）。此外，捕获的癌细胞的存活率保持在 84% 以上。该方法可有效地用于捕获高效率和高纯度的 CTC，用于癌症状态的诊断和监测。
Circulating tumor cells (CTCs) have attracted considerable attention as promising markers for diagnosing and monitoring the cancer status. Despite many technological advances in isolating CTCs, the capture efficiency and purity still remain challenges that limit clinical practice. Here, the construction of "nanotentacle"-structured magnetic particles using M13-bacteriophage and their application for the efficient capturing of CTCs is demonstrated. The M13-bacteriophage to magnetic particles followed by modification with PEG is conjugated, and further tethered monoclonal antibodies against the epidermal receptor 2 (HER2). The use of nanotentacle-structured magnetic particles results in a high capture purity (>45%) and efficiency (>90%), even for a smaller number of cancer cells (approximate to 25 cells) in whole blood. Furthermore, the cancer cells captured are shown to maintain a viability of greater than 84%. The approach can be effectively used for capturing CTCs with high efficiency and purity for the diagnosis and monitoring of cancer status.