Transcription of hepatitis B virus in peripheral blood mononuclear cells from persistently infected patients
Transcription of hepatitis B virus in peripheral blood mononuclear cells from persistently infected patients
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DOI：
10.1128/jvi.71.7.5399-5407.1997
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发表时间：
1997-07-01
影响因子：
5.4
通讯作者：
Gerlich, WH
中科院分区：
文献类型：
--
作者：
StollBecker, S;Repp, R;Gerlich, WH
据报道，乙型肝炎病毒（HBV）存在于外周血单核细胞（PBMC）中，但尚不清楚它是否在那里复制。复制的先决条件应该是共价闭合病毒 DNA 的形成和所有必需病毒 mRNA 的转录。 HBV 的 mRNA 形成具有共同 3' 末端的嵌套盒。为了检测甚至低水平的潜在复制，我们开发了一种定量逆转录 PCR 方法，用于在存在较大 HBV mRNA 的情况下检测较小的 HBV mRNA 种类。迄今为止测试的所有三名高病毒血症患者的 PBMC 中均含有病毒大、小表面蛋白和 X 蛋白的 mRNA，但其血清中的病毒中却没有。此外，我们通过 PCR 在其 PBMC 中检测到共价闭合病毒 DNA。这些数据表明，乙型肝炎病毒不仅可以被单核血细胞吸收，还可以被单核血细胞复制，并且这些细胞可能是病毒持续存在的肝外部位。 X mRNA 的检测量最大。 X蛋白可能会干扰单核细胞在针对病毒的免疫反应过程中的功能。
Hepatitis B virus (HBV) has been reported to exist in peripheral blood mononuclear cells (PBMC), but it is not clear whether it replicates there. A precondition for replication should be the formation of covalently closed viral DNA and transcription of all essential viral mRNAs. The mRNAs of HBV form a nested box with common 3' ends. In order to detect even low levels of potential replication, we developed a quantitative reverse transcription-PCR method for detection of a smaller HBV mRNA species in the presence of the larger ones. All three highly viremic patients tested so far had mRNAs for the large and the small surface proteins and the X protein of the virus within PBMC but not in the virus from their sera. Furthermore, we detected by PCR covalently closed viral DNA in their PBMC. These data suggest that HBV may be not only taken up but also replicated by mononuclear blood cells and that these cells may be an extrahepatic site of viral persistence. X mRNA was detected in the largest amount. Possibly, X protein interferes with functions of the mononuclear cells during the immune response against the virus.