OLEOCANTHAL AMELIORATES AMYLOID-β OLIGOMERS' TOXICITY ON ASTROCYTES AND NEURONAL CELLS: IN VITRO STUDIES
OLEOCANTHAL AMELIORATES AMYLOID-β OLIGOMERS' TOXICITY ON ASTROCYTES AND NEURONAL CELLS: IN VITRO STUDIES
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DOI：
10.1016/j.neuroscience.2017.03.059
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发表时间：
2017-06-03
期刊：
影响因子：
3.3
通讯作者：
Kaddoumi, Amal
中科院分区：
文献类型：
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作者：
Batarseh, Yazan S.;Mohamed, Loqman A.;Kaddoumi, Amal
特级初榨橄榄油（EVOO）具有多种健康促进作用。有证据表明，EVOO在实验动物模型中减弱了淀粉样蛋白β（A β）的病理学并改善了认知功能，这表明它有可能保护和降低患阿尔茨海默病（AD）的风险。现有的研究已经将这种有益效果与EVOO中的活性成分之一oleocanthal联系起来。油珊瑚醛对AD病理学的作用与其在体外减弱A β和tau聚集的能力以及在体内增强A β从野生型和AD转基因小鼠的脑中清除的能力有关。然而，oleocanthal改变A β对脑实质细胞的毒性作用的能力是未知的。在本研究中，我们研究了油刺对神经元和星形胶质细胞中A β寡聚体（A β o）病理事件的调节作用。我们的研究结果表明，oleocanthal阻止A β o诱导的突触蛋白，SNAP-25和PSD-95，下调神经元，并减弱A β o诱导的炎症，谷氨酰胺转运蛋白（GLT 1）和葡萄糖转运蛋白（GLUT 1）下调星形胶质细胞。A β 0诱导的炎症的特征在于白细胞介素-6（IL-6）增加和胶质细胞酸性蛋白（GFAP）上调，其被油皮醛减少。总之，这项研究提供了进一步的证据，支持EVOO衍生的酚开环环烯醚萜油珊瑚醛对AD病理的保护作用。(C)2017年IBRO。由爱思唯尔有限公司出版。保留所有权利。
Extra-virgin olive oil (EVOO) has several health promoting effects. Evidence have shown that EVOO attenuates the pathology of amyloid-beta (A beta) and improves cognitive function in experimental animal models, suggesting it's potential to protect and reduce the risk of developing Alzheimer's disease (AD). Available studies have linked this beneficial effect to oleocanthal, one of the active components in EVOO. The effect of oleocanthal against AD pathology has been linked to its ability to attenuate A beta and tau aggregation in vitro, and enhance A beta clearance from the brains of wild-type and AD transgenic mice in vivo. However, the ability of oleocanthal to alter the toxic effect of A beta on brain parenchymal cells is unknown. In the current study, we investigated oleocanthal effect on modulating A beta oligomers (A beta o) pathological events in neurons and astrocytes. Our findings demonstrated oleocanthal prevented A beta o-induced synaptic proteins, SNAP-25 and PSD-95, down-regulation in neurons, and attenuated A beta o-induced inflammation, glutamine transporter (GLT1) and glucose transporter (GLUT1) down-regulation in astrocytes. A beta o-induced inflammation was characterized by interleukin-6 (IL-6) increase and glial fibrillary acidic protein (GFAP) upregulation that were reduced by oleocanthal. In conclusion, this study provides further evidence to support the protective effect of EVOO-derived phenolic secoiridoid oleocanthal against AD pathology. (C) 2017 IBRO. Published by Elsevier Ltd. All rights reserved.