Combined Salvianolic Acid B and Ginsenoside Rg1 Exerts Cardioprotection against Ischemia/Reperfusion Injury in Rats
Combined Salvianolic Acid B and Ginsenoside Rg1 Exerts Cardioprotection against Ischemia/Reperfusion Injury in Rats
复制标题
丹酚酸 B 和人参皂苷 Rg1 组合对大鼠缺血/再灌注损伤具有心脏保护作用
DOI：
10.1371/journal.pone.0135435
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发表时间：
2015-08-17
期刊：
影响因子：
3.7
通讯作者：
Jiang, Baohong
中科院分区：
文献类型：
--
作者：
Deng, Yanping;Yang, Min;Jiang, Baohong
临床上药物治疗策略的缺乏限制了心肌缺血/再灌注（I/R）损伤患者的预后。本研究旨在评价丹参酮酸B（SalB）和丹参素Rg 1（Rg 1）联合应用对心肌I/R损伤的保护作用，并进一步探讨其机制。结扎Wistar雄性大鼠冠状动脉造成I/R损伤，H9 c2细胞造成缺氧/复氧损伤。首先，通过血流动力学测定，确定SalB与Rg 1的最佳配比为2：5。SalB-Rg 1（2：5）能维持H9 c2细胞缺氧/复氧损伤时线粒体膜电位，对抗细胞凋亡和坏死。与相同剂量的SalB或Rg 1相比，SalB-Rg 1在降低心肌梗死面积、维持心肌结构、改善心功能、减少TNF-α、IL-1β、RANTES和sVCAM-1等细胞因子分泌方面的作用更为显著。最后，使用流式细胞术，SalB-Rg 1改善了I/R损伤大鼠心肌细胞而不是心脏成纤维细胞的活力。我们的研究结果表明，SalB-Rg 1是一个有前途的策略，以防止心肌I/R损伤。
Lack of pharmacological strategies in clinics restricts the patient prognosis with myocardial ischemia/reperfusion (I/R) injury. The aim of this study was to evaluate the cardioprotection of combined salvianolic acid B (SalB) and ginsenoside Rg1 (Rg1) against myocardial I/R injury and further investigate the underlying mechanism. I/R injury was induced by coronary artery ligation for Wistar male rats and hypoxia/reoxygenation injury was induced on H9c2 cells. Firstly, the best ratio between SalB and Rg1was set as 2:5 based on their effects on heart function detected by hemodynamic measurement. Then SalB-Rg1 (2:5) was found to maintain mitochondrial membrane potential and resist apoptosis and necrosis in H9c2 cell with hypoxia/reoxygenation injury. Companying with same dose of SalB or Rg1 only, SalB-Rg1 showed more significant effects on down-regulation of myocardial infarct size, maintenance of myocardium structure, improvement on cardiac function, decrease of cytokine secretion including TNF-α, IL-1β, RANTES and sVCAM-1. Finally, the SalB-Rg1 improved the viability of cardiac myocytes other than cardiac fibroblasts in rats with I/R injury using flow cytometry. Our results revealed that SalB-Rg1 was a promising strategy to prevent myocardial I/R injury.