Up-conversion fluorescence biosensor for sensitive detection of CA-125 tumor markers
Up-conversion fluorescence biosensor for sensitive detection of CA-125 tumor markers
复制标题
用于灵敏检测 CA-125 肿瘤标志物的上转换荧光生物传感器
DOI：
10.1016/j.jre.2019.03.018
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发表时间：
2019-09-01
影响因子：
4.9
通讯作者：
Hong, Decheng
中科院分区：
文献类型：
--
作者：
Liu, Jingshi;Xu, Sai;Hong, Decheng
肿瘤标志物的敏感性和特异性生物测定对于早期癌症检测和治疗至关重要。近年来，镧系元素（Ln（3+））掺杂的上转换发光纳米粒子（UCNPs）在肿瘤标志物检测方面受到广泛关注。本文中，我们将NaYF 4：Yb、Tm和银纳米颗粒分别作为能量供体和受体组合，以形成基于上转换荧光的抑制性肿瘤标志物生物传感器系统。用银纳米粒子标记肿瘤标志物CA-125分子，并且可以检测UCNP与银纳米粒子受体之间的能量转移荧光信号。该生物传感器在5 ~ 100 ng/mL浓度范围内具有良好的稳定性、灵敏度和选择性，检测限为120 pg/mL。该技术具有制作简单、操作方便、成本低、信息容量大等优点，在临床应用中具有很大的潜力。(C)2019中国稀土学会Elsevier B. V.出版，保留所有权利。
Sensitive and specific bioassays of tumor markers are critical for early cancer detection and treatment. In recent years, lanthanide (Ln(3+)) doped upconversion nanoparticles (UCNPs) have attracted wide attentions in tumor markers detection. Herein, we combined NaYF4:Yb,Tm and silver nanoparticles, serving as energy donor and receptor, respectively, to form an up-conversion fluorescence based inhibitory tumor marker biosensor system. The tumor marker CA-125 molecules are labeled with silver NPs, and the energy transfer fluorescent signal can be detected between the UCNPs and the silver NP receptors. The biosensor shows good stability, high sensitivity and selectivity in the tumor marker concentration range from 5 to 100 ng/mL, as well as a detection limit of 120 pg/mL. Due to the advantage of ease of fabrication and operation, low cost and high information capacity, this technology holds great potential for the clinical applications. (C) 2019 Chinese Society of Rare Earths. Published by Elsevier B.V. All rights reserved.