TERE;: a novel cis-element responsible for a coordinated expression of genes related to programmed cell death and secondary wall formation during differentiation of tracheary elements
TERE;: a novel cis-element responsible for a coordinated expression of genes related to programmed cell death and secondary wall formation during differentiation of tracheary elements
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DOI：
10.1111/j.1365-313x.2007.03180.x
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发表时间：
2007-09-01
期刊：
影响因子：
7.2
通讯作者：
Fukuda, Hiroo
中科院分区：
文献类型：
--
作者：
Pyo, Hyunjin;Demura, Taku;Fukuda, Hiroo
导水管元件（TE）的分化是次生壁结构精心构建的结果，包括木质化和程序性细胞死亡（PCD），包括细胞自溶。为了了解 TE 分化的精心调控，我们研究了指导 TE 分化的基因表达的调控机制。对赋予 TE 特异性表达的 ZCP4（Zinniacysteine 蛋白酶 4）启动子进行详细的功能丧失和功能获得分析，证明新的 11 bp 顺式元件对于未成熟的 TE 特异性启动子活性是必要且充分的。在许多拟南芥TE分化相关基因的启动子中发现了11-bp顺式元件样序列。对来自次生壁形成或修饰相关基因以及 PCD 相关基因的类似推定顺式元件进行的功能获得分析表明，顺式元件也足以用于基因的 TE 特异性表达。这些结果表明，被称为气管元件调节顺式元件的共同序列赋予与次生壁形成或修饰和PCD相关的两个基因TE特异性表达。
The differentiation of water-conducting tracheary elements (TEs) is the result of the orchestrated construction of secondary wall structure, including lignification, and programmed cell death (PCD), including cellular autolysis. To understand the orchestrated regulation of differentiation of TEs, we investigated the regulatory mechanism of gene expression directing TE differentiation. Detailed loss-of-function and gain-of-function analyses of the ZCP4 (Zinniacysteine protease 4) promoter, which confers TE-specific expression, demonstrated that a novel 11-bp cis-element is necessary and sufficient for the immature TE-specific promoter activity. The 11-bp cis-element-like sequences were found in promoters of many Arabidopsis TE differentiation-related genes. A gain-of-function analysis with similar putative cis-elements from secondary wall formation or modification-related genes as well as PCD-related genes indicated that the cis-elements are also sufficient for TE-specific expression of genes. These results demonstrate that a common sequence, designated as the tracheary-element-regulating cis-element, confers TE-specific expression to both genes related to secondary wall formation or modification and PCD.