Distinct changing profiles of hepatitis A and E virus infection among patients with acute hepatitis in Mongolia: The first report of the full genome sequence of a novel genotype 1 hepatitis E virus strain
Distinct changing profiles of hepatitis A and E virus infection among patients with acute hepatitis in Mongolia: The first report of the full genome sequence of a novel genotype 1 hepatitis E virus strain
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DOI：
10.1002/jmv.24907
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发表时间：
2018-01-01
影响因子：
12.7
通讯作者：
Okamoto, Hiroaki
中科院分区：
文献类型：
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作者：
Tsatsralt-Od, Bira;Primadharsini, Putu Prathiwi;Okamoto, Hiroaki
2012年1月，蒙古启动了一项甲型肝炎疫苗接种计划，该计划尚未得到评估。2013年首次发生由基因4型戊型肝炎病毒（HEV）引起的本地急性戊型肝炎，这表明有必要进行常规研究以监测其流行情况。研究了2014年至2015年间蒙古乌兰巴托连续154例临床诊断为急性肝炎的患者。通过血清学和分子检测、测序和系统发育分析，只有1例（0.6%）被诊断为由基因型A型肝炎病毒（HAV）引起的急性A型肝炎，32例（20.8%）被诊断为由基因型1型HEV引起的急性E型肝炎。本研究获得的32株HEV分离株核苷酸同源性为99.5-100%，与1a - 1f亚型分离。在全基因组的p-距离比较中，一个代表性HEV分离株（MNE15-072）与1a-1f株之间的距离为0.071 ~ 0.137,1b与1c之间的距离为0.062 ~ 0.070。总之，自疫苗接种计划开始以来，蒙古的急性甲型肝炎患病率有所下降，而一种可能是新型亚型的单系基因1型HEV毒株一直很流行。
In January 2012, Mongolia started a hepatitis A vaccination program, which has not yet been evaluated. The first occurrence of autochthonous acute hepatitis E in 2013, caused by genotype 4 hepatitis E virus (HEV), suggests the need for a routine study to monitor its prevalence. One hundred fifty-four consecutive patients who were clinically diagnosed with acute hepatitis between 2014 and 2015 in Ulaanbaatar, Mongolia were studied. By serological and molecular testing followed by sequencing and phylogenetic analysis, only one patient (0.6%) was diagnosed with acute hepatitis A, caused by genotype IA hepatitis A virus (HAV), and 32 (20.8%) patients were diagnosed with acute hepatitis E, caused by genotype 1 HEV. The 32 HEV isolates obtained in this study shared 99.5-100% nucleotide identity and were grouped into a cluster separated from those of subtypes 1a to 1f. Upon comparison of p-distances over the entire genome, the distances between one representative HEV isolate (MNE15-072) and 1a-1f strains were 0.071-0.137, while those between 1b and 1c were 0.062-0.070. In conclusion, the prevalence of acute hepatitis A has decreased in Mongolia since the start of the vaccination program, while the monophyletic genotype 1 HEV strain of a probably novel subtype has been prevalent.