Employment of 16S rDNA gene sequencing techniques for improved identification of difficult-to-identify bacterial veterinary pathogens
Employment of 16S rDNA gene sequencing techniques for improved identification of difficult-to-identify bacterial veterinary pathogens
复制标题
采用 16S rDNA 基因测序技术改进难以识别的细菌性兽医病原体的识别
DOI：
--
复制
发表时间：
2008
期刊：
影响因子：
--
通讯作者：
M. Matsuda
中科院分区：
文献类型：
--
作者：
J. Moore;Y. Maeda;Jiru Xu;B. C. Millar;Peter H. Herold;V. M. J. Browne;C. Goldsmith;A. Loughrey;P. Rooney;J. Elborn;M. Matsuda
采用16S rDNA PCR和自动测序技术鉴定来自禽类、马、犬和绵羊来源的细菌性兽医病原体，这些病原体已被证明难以采用常规培养技术进行鉴定。对来自临床兽医标本的46种难以识别的细菌分离株进行了通用或“宽范围”真细菌PCR。使用两套通用引物进行16S rDNA PCR，成功地产生了1，068 bp的复合扩增子，将其测序以获得每个分离物的身份。序列分析能够相对容易地鉴定所有检测的分离株。当使用分子鉴定方法是合理的，例如在动物健康的爆发控制或生物恐怖主义中，使用部分16S rDNA PCR和使用通用或“宽范围”16S rDNA的测序，提供了鉴定此类病原体的有价值和可靠的方法。
To employ 16S rDNA PCR and automated sequencing techniques to identify a collection of bacterial veterinary pathogens from avian, equine, canine and ovine sources, that have proven difficult to identify, employing conventional cultural techniques. Universal or “broad-range” eubacterial PCR was performed on a collection of 46 difficult-to-identify bacterial isolates originating from clinical veterinary specimens. 16S rDNA PCR was performed using two sets of universal primers to successfully generate a composite amplicon of 1,068 bp, which was sequenced to obtain each isolate’s identity. Sequence analysis was able to identify all isolates examined with relative ease. Where the use of molecular identification methods is justified, such as in outbreak control or bioterrorism in animal health, employment of partial 16S rDNA PCR and sequencing employing universal or “broad-range” 16S rDNA, provides a valuable and reliable method of identification of such pathogens.