Construction of cellulose nanofibers/quaternized chitin/organic rectorite composites and their application as wound dressing materials
Construction of cellulose nanofibers/quaternized chitin/organic rectorite composites and their application as wound dressing materials
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纤维素纳米纤维/季铵化甲壳素/有机累托石复合材料的构建及其作为伤口敷料材料的应用
DOI：
10.1039/c9bm00288j
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发表时间：
2019-06-01
影响因子：
6.6
通讯作者：
Zhang, Lina
中科院分区：
文献类型：
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作者：
Gao, Huimin;Zhong, Zibiao;Zhang, Lina
创伤性损伤是死亡的主要原因，伤口愈合不良影响着数百万人。因此，开发有效的伤口敷料对于加速伤口愈合和降低死亡率至关重要。在这项研究中，具有高纵横比的羧化褐藻纤维素纳米纤维（BACNF）的悬浮液被冷冻干燥以制备海绵。该海绵显示出高孔隙率和吸水能力;因此，当用作伤口敷料时，其可以吸收伤口渗出物。此外，将具有抗菌性能的季铵化壳聚糖（QC）通过静电相互作用插层到有机累托石（OREC）的层间，得到了比单独使用QC具有更高抗菌活性的复合悬浮液（QCR）。然后将BACNF海绵浸泡在QCR悬浮液中，通过静电作用和氢键作用吸附QCR，通过冷冻干燥法制备了纤维素/季铵化甲壳素/有机累托石复合海绵（BACNF/QCR）。体内动物试验表明，BACNF/QCR海绵在大鼠断尾试验中迅速诱导止血，使其上级优于传统止血材料。此外，BACNFs/QCRs可以显著促进胶原合成和新生血管形成，从而比纱布提前3天加速伤口愈合。这种使用天然物质制造的多功能生物医学材料显示出用于伤口愈合的巨大潜力。
Traumatic injury is a major cause of mortality, and poor wound healing affects millions of people. Thus, the development of effective wound dressings is essential for speeding up wound healing and decreasing mortality. In this study, a suspension of carboxylated brown algae cellulose nanofibers (BACNFs) with a high aspect ratio was freeze dried to prepare a sponge. The sponge showed high porosity and water absorption capacity; thus, it can absorb wound exudates when used as a wound dressing. In addition, quaternized ss-chitin (QC) with antibacterial properties was intercalated into the interlayer space of the organic rectorite (OREC) via electrostatic interactions to obtain composite suspensions (QCRs) with improved antimicrobial activity compared to that of QC alone. Subsequently, the BACNF sponge was soaked in the QCR suspension to absorb QCRs via electrostatic interactions and hydrogen bonding from which cellulose nanofiber/quaternized chitin/organic rectorite composite (BACNF/QCR) sponges were constructed via freeze-drying. The in vivo animal tests demonstrated that the BACNF/QCR sponges rapidly induced hemostasis in a rat tail amputation test, making them superior to the traditional hemostatic materials. Furthermore, BACNFs/QCRs could substantially promote collagen synthesis and neovascularization, thereby accelerating wound healing 3 days earlier than gauze. This multi-functional biomedical material, fabricated using natural substances, shows great potential to be used for wound healing.