NUCLEOSIDE TRIPHOSPHATE TERMINI FROM RNA SYNTHESIZED IN VIVO BY ESCHERICHIA COLI
NUCLEOSIDE TRIPHOSPHATE TERMINI FROM RNA SYNTHESIZED IN VIVO BY ESCHERICHIA COLI
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DOI：
10.1126/science.164.3883.1067
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发表时间：
1969-01-01
期刊：
影响因子：
56.9
通讯作者：
NIERLICH, DP
中科院分区：
文献类型：
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作者：
JORGENSEN, SE;BUCH, LB;NIERLICH, DP
大肠杆菌ribonucleoside-3′-monophosphate-5′-triphosphates.体内合成核糖核酸的碱性水解物这可能是由于新生的RNA链5‘端的初始核苷三磷酸的水解所致。对数生长的培养物，用32P标记的磷酸盐标记45秒，每个细胞产生大约2000个标记的四磷酸盐分子，随着继续标记，产量只略有增加。在大肠杆菌中只发现了腺苷和鸟苷的四磷酸盐，而且这两种物质的含量大致相同。
Alkaline hydrolyzates of RNA made in vivo byEscherichia colicontain ribonucleoside-3′-monophosphate-5′-triphosphates. These probably arise by hydrolysis of the initial nucleoside triphosphate from the 5′ terminus of the nascent RNA chains. Logarithmically growing cultures, labeled for 45 seconds with32P-labeled phosphate, yield about 2000 molecules of labeled tetraphosphate per cell, this yield increasing only slightly with continued labeling. Only the tetraphosphates of adenosine and guanosine have been found inEscherichia coli, and these two are present in approximately equal amounts.