CLONING AND EXPRESSION OF FULL-LENGTH CDNA-ENCODING HUMAN VITAMIN-D RECEPTOR
CLONING AND EXPRESSION OF FULL-LENGTH CDNA-ENCODING HUMAN VITAMIN-D RECEPTOR
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DOI：
10.1073/pnas.85.10.3294
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发表时间：
1988-05-01
影响因子：
11.1
通讯作者：
OMALLEY, BW
中科院分区：
文献类型：
--
作者：
BAKER, AR;MCDONNELL, DP;OMALLEY, BW
已从人肠和T47 D细胞cDNA文库中分离出编码人维生素D受体的互补DNA克隆。4605个碱基对（bp）的cDNA的核苷酸序列包括115 bp的非编码前导序列、1281 bp的开放阅读框架和3209 bp的3“非编码序列。两个聚腺苷酸化信号AATAAA分别存在于poly（A）尾上游25和70 bp处。RNA印迹杂交表明单个mRNA种类。4600 bp。将克隆序列转染入COS-1细胞导致产生与天然受体无区别的单一受体种类。序列比较表明，维生素D受体属于类固醇受体基因家族，在大小和序列上与该家族的另一个成员甲状腺激素受体最接近。
Complementary DNA clones encoding the human vitmain D receptor have been isolated from human intestine and T47D cell cDNA libraries. The nucleotide sequence of the 4605-base pair (bp) cDNA includes a noncoding leader sequence of 115 bp, a 1281-bp open reading frame, and 3209 bp of 3'' noncoding sequence. Two polyadenylylation signals, AATAAA, are present 25 and 70 bp upstream of the poly(A) tail, respectively. RNA blot hybridization indicates a single mRNA species of .apprxeq. 4600 bp. Transfection of the clonedsequences into COS-1 cells results in the production of a single receptor species indistinguishable from the native receptor. Sequence comparisons demonstrate that the vitamin D receptor belongs to the steroid-receptor gene family and is closest in size and sequence to another member of this family, the thyroid hormone receptor.