Identification of a paternal developmental effect on the cytoplasm of one-cell-stage mouse embryos.
Identification of a paternal developmental effect on the cytoplasm of one-cell-stage mouse embryos.
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鉴定父系发育对单细胞阶段小鼠胚胎细胞质的影响。
DOI：
10.1073/pnas.83.18.6883
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发表时间：
1986
影响因子：
11.1
通讯作者：
C. Babinet
中科院分区：
文献类型：
--
作者：
J. Renard;C. Babinet
雌性DDK小鼠与BALB/c品系雄性小鼠的交配是不育的，而正反交通常是可育的。我们使用这两个品系的杂交卵子之间的核移植来研究这种效应的基础。我们证明，观察到的不育是早期胚胎死亡的结果，死亡是由于卵细胞质的修饰，并且这种修饰是由男性原核介导的。一旦确定，这种修饰可能会影响C57BL/6等无关基因的女性原核。这些结果支持这样的观点，即来自男性基因组的产物作用于原核阶段，并可能影响胚胎发育的后期阶段。
Matings of female DDK mice with males of the BALB/c strain are sterile, whereas reciprocal crosses are normally fertile. We used nuclear transplantation between the hybrid eggs of these two strains to investigate the basis of this effect. We demonstrate that the observed sterility results from early embryonic mortality, that the mortality is due to a modification of the egg cytoplasm, and that the modification is mediated by the male pronucleus. Once established, this modification may affect female pronuclei of unrelated genotype such as C57BL/6. These results support the notion that a product derived from the male genome acts at the pronuclear stage and can affect later stages of embryonic development.