Depletion of the photosystem II core complex in mature tobacco leaves infected by the Flavum strain of tobacco mosaic virus
Depletion of the photosystem II core complex in mature tobacco leaves infected by the Flavum strain of tobacco mosaic virus
复制标题
DOI：
10.1094/mpmi.2003.16.12.1135
复制
发表时间：
2003-12-01
影响因子：
3.5
通讯作者：
Aro, EM
中科院分区：
文献类型：
--
作者：
Lehto, K;Tikkanen, M;Aro, EM
烟草花叶病毒（TMV）的黄株与野生型（wt）病毒的不同之处在于在系统感染的发育中的叶片中引起强烈的黄色和绿色花叶，在完全展开的叶片中变黄，以及在两种类型的感染组织中叶绿体的明显畸形。分析的类囊体蛋白的flavum株感染的烟草叶片表明，在成熟叶片中的褪绿伴随着整个光系统11（PSII）的核心复合物和33 kDa的蛋白质的放氧复合物的耗尽。在相应的野生型TMV感染叶片的类囊体蛋白中观察到的唯一变化是ATP合酶复合物的α和β亚基的轻微减少。已知TMV不同黄化株系的外壳蛋白在叶绿体内有效积累，但在这项工作中，还检测到病毒运动蛋白与黄株系感染叶片的类囊体膜相关。叶绿素生物合成途径中不同酶的mRNA在成熟的褪绿叶片中没有减少。这些结果表明，褪绿不是由于色素生物合成的减少，而是由于PSII核心复合物的特定蛋白质的减少和随后的色素分解引起的。
The flavum strain of Tobacco mosaic virus (TMV) differs from the wild-type (wt) virus by causing strong yellow and green mosaic in the systemically infected developing leaves, yellowing in the fully expanded leaves, and distinct malformations of chloroplasts in both types of infected tissues. Analysis of the thylakoid proteins of flavum strain-infected tobacco leaves indicated that the chlorosis in mature leaves was accompanied by depletion of the entire photosystem 11 (PSII) core complexes and the 33-kDa protein of the oxygen evolving complex. The only change observed in the thylakoid proteins of the corresponding wt TMV-infected leaves was a slight reduction of the alpha and beta subunits of the ATP synthase complex. The coat proteins of different yellowing strains of TMV are known to effectively accumulate inside chloroplasts, but in this work, the viral movement protein also was detected in association with the thylakoid membranes of flavum strain-infected leaves. The mRNAs of different enzymes involved in the chlorophyll biosynthesis pathway were not reduced in the mature chlorotic leaves. These results suggest that the chlorosis was not caused by reduction of pigment biosynthesis, but rather, by reduction of specific proteins of the PSII core complexes and by consequent break-down of the pigments.