ENHANCED SUSCEPTIBILITY TO HPD-SENSITIZED PHOTOTOXICITY AND CORRELATED RESISTANCE TO TRYPSIN DETACHMENT IN SV40-TRANSFORMED IMR-90 CELLS
ENHANCED SUSCEPTIBILITY TO HPD-SENSITIZED PHOTOTOXICITY AND CORRELATED RESISTANCE TO TRYPSIN DETACHMENT IN SV40-TRANSFORMED IMR-90 CELLS
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DOI：
10.1111/j.1751-1097.1986.tb09506.x
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发表时间：
1986-02-01
影响因子：
3.3
通讯作者：
WILLIAMS, JR
中科院分区：
文献类型：
--
作者：
DENSTMAN, SC;DILLEHAY, LE;WILLIAMS, JR
用血卟啉衍生物（HPD）和红光对人正常细胞株IMR-90及其衍生细胞株SV 40转化的反应进行了比较。当通过克隆形成存活率测定时，转化细胞以比正常细胞显著更高的速率失活。用HPD和光共处理还诱导细胞对胰蛋白酶的酶裂解的抗性;转化细胞表现出比正常细胞更大的对从生长表面脱离的抗性。这些数据表明，转化细胞可能具有内在的特性，使他们比正常细胞更敏感的HPD诱导的光毒性和质膜可能是网站的差异毒性。
The response of a normal human cell strain, IMR-90 and a line derived from it by SV40 transformation were compared after treatment in vitro with hematoporphyrin derivative (HPD) and red light. Transformed cells were inactivated at a significantly higher rate than normal cells when assayed by clonogenic survival. Co-treatment with HPD and light also induces cellular resistance to enzymatic cleavage by trypsin; transformed cells exhibit a greater resistance than the normal cells to detachment from the growing surface. These data indicate that transformed cells may possess intrinsic characteristics that render them more sensitive than normal cells to HPD-induced phototoxicity and that the plasma membrane may be the site of differential toxicity.