Circulating dengue virus serotypes in Bangladesh from 2013 to 2016
Circulating dengue virus serotypes in Bangladesh from 2013 to 2016
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DOI：
10.1007/s13337-018-0469-x
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发表时间：
2018-09-01
期刊：
影响因子：
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通讯作者：
Shirin, Tahmina
中科院分区：
文献类型：
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作者：
Muraduzzaman, A. K. M.;Alam, Ahmed Nawsher;Shirin, Tahmina
为了确定孟加拉国登革热病毒的流行血清型，对2013-2016年期间登革热监测的储存血清样本进行了回顾性分子鉴定。采用真实的RT-PCR方法检测发热5天以内患者血清中登革病毒核酸。对登革PCR阳性的标本进一步进行真实的时间RT-PCR血清型分析。登革病毒感染的总体流行率在研究年份的13-42%之间变化，4月至9月为单峰。在四种登革热血清型中，DEN 1和DEN 2在三个大城市的循环中相继出现DEN 1，其中DEN 2在研究期间持续存在。在邻国，所有四种血清型的持续存在使孟加拉国容易受到破坏性的二次感染，因为除了目前在该国流行的病毒之外，还引入了新的血清型。因此，持续的病毒学监测对于早期预警新血清型在流通中的出现和公共卫生准备至关重要。
To identify the circulating serotype(s) of dengue viruses in Bangladesh, a retrospective molecular identification was performed on stored serum samples of dengue surveillance during the period of 2013-2016. Real time RT-PCR was performed on serum samples collected from the patients with less than 5 days fever for detection of dengue virus nucleic acid. The samples, positive for dengue PCR were further analyzed for serotypes by real time RT-PCR. The overall prevalence of dengue virus infection was varied among 13-42% in study years with a single peak flanked by April to September. Among the four dengue serotypes DEN1 and DEN2 were in the circulation in three metropolitan cities with sequential emergence of DEN1 where DEN2 was persisted constantly during the study period. Persistence of all four serotypes in the neighboring country makes Bangladesh vulnerable for devastating secondary infection by introduction of new serotype(s) other than currently circulating viruses in the country. Thus continuous virological surveillance is crucial for early warning of emergence of new serotype in the circulation and public health preparedness.