Potentials of mouthwashes in disinfecting cariogenic bacteria and biofilms leading to inhibition of caries.
Potentials of mouthwashes in disinfecting cariogenic bacteria and biofilms leading to inhibition of caries.
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DOI：
10.2174/1874210601206010023
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发表时间：
2012
期刊：
影响因子：
--
通讯作者：
Matin K
中科院分区：
文献类型：
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作者：
Oyanagi T;Tagami J;Matin K
本研究的目的是比较某些市售漱口水在获得龋齿抑制潜力后对致龋细菌和生物膜的影响。对含有 I) 葡萄糖酸氯己定 (CHG；0.0005% w/v)、II) 苄索氯铵 (BTC；0.01% w/v)、III) 精油（李施德林）和 IV) 聚维酮碘 (PVP-I；0.035% w/v) 的漱口水进行了浮游致龋细菌、生物膜和离体龋齿模型的测试。分别用每种溶液接种细菌等分试样并在室温下涡旋10秒。随后用 BacLight 活力试剂盒染色后通过荧光显微镜 (FM) 研究细菌活力，并计算菌落形成单位 (CFU) 的数量。类似地，将漱口溶液应用于人工致龋生物膜，并如上所述研究生物膜的细菌活力。使用生物膜诱导的龋齿和继发性龋齿模型研究了两种选定的漱口水对龋齿病变的抑制潜力。在所有步骤中，都包含磷酸盐缓冲盐水 (PBS) 溶液作为对照。与 PBS 和其他漱口剂相比，李施德林和 PVP-I 处理可杀死大量浮游致龋菌和生物膜中的细菌。与其他溶液相比，用李施德林和 PVP-I 处理后，CFU 计数也显着减少（P<0.05）。李施德林在防止脱矿进展方面也表现出显着的抑制作用（P<0.05）。漱口水的杀菌效力差异很大，这表明李施德林等漱口水可用于预防龋齿和继发性龋齿。
The aim of this study was to compare the effects of certain commercially available mouthwashes on cariogenic bacteria and biofilms, following the acquisition of inhibition potentials of caries. Mouthwashes containing I) chlorhexidine gluconate (CHG; 0.0005% w/v), II) benzethonium chloride (BTC; 0.01% w/v), III) an essential oil (Listerine), and IV) povidone-iodine (PVP-I; 0.035% w/v) were tested on planktonic cariogenic bacteria, biofilms, and an ex vivo caries model. Bacterial aliquots were inoculated with each solution separately and vortexed for 10 seconds at room temperature. Bacterial viability was subsequently investigated by fluorescence microscopy (FM) after staining with a BacLight viability kit and the number of colony-forming units (CFUs) was counted. Similarly, mouthwash solutions were applied to artificial cariogenic biofilms, and bacterial viability of the biofilms was investigated as stated above. Inhibition potentials of two selected mouthwashes of carious lesions were investigated using biofilm-induced caries and a secondary caries model. In all steps, a phosphate-buffered saline (PBS) solution was included as a control. Planktonic cariogenic bacteria and bacteria embedded in biofilms were killed in remarkably large numbers with Listerine and PVP-I treatment compared to PBS and other gargles. CFU counts also showed significant reduction after treatment with Listerine and PVP-I compared to other solutions (P<0.05). Listerine also displayed significant (P<0.05) inhibition effects in preventing the progression of demineralization. Bactericidal potencies of the mouthwashes varied significantly, suggesting that mouthwashes like Listerine can be useful for the prevention of caries and secondary caries.