A genetic analysis of tetrahymena that have aborted normal development.
A genetic analysis of tetrahymena that have aborted normal development.
复制标题
对已中止正常发育的四膜虫进行遗传分析。
DOI：
10.1093/genetics/102.1.29
复制
发表时间：
1982
期刊：
影响因子：
3.3
通讯作者：
Bruns,PJ
中科院分区：
文献类型：
--
作者：
Scholnick,SB;Bruns,PJ
接合四膜虫可以在产生后代体细胞（大）核并恢复营养生长之前中止性周期。我们分析了这些细胞的生发（微核）基因型，以确定它们中止接合的阶段。我们的数据表明，几乎所有这些细胞都尝试减数分裂，但很少有细胞能够成功完成异体受精。由此产生的微核基因型表明，在减数分裂或异体受精失败的尝试中，生发染色体或整个细胞核都丢失了。我们的结论是，发育后代大核的决定是在减数分裂期间做出的，并且取决于成功异体受精所需的某些步骤的完成。
Conjugating Tetrahymena can abort the sexual cycle before the production of progeny somatic (macro-) nuclei and return to vegetative growth. We have analyzed the germinal (micronuclear) genotypes of these cells in order to determine the stage at which they aborted conjugation. Our data demonstrate that nearly all of these cells attempt meiosis, but that very few reach the successful completion of cross-fertilization. The resulting micronuclear genotypes suggest that either germinal chromosomes or entire nuclei are lost during an unsuccessful attempt at meiosis or cross-fertilization. We conclude that the decision to develop progeny macronuclei is made during meiosis and is dependent on the completion of some step necessary for successful cross-fertilization.