Relationship between altered expression levels of MIR21, MIR143, MIR145, and MIR205 and clinicopathologic features of esophageal squamous cell carcinoma
Relationship between altered expression levels of MIR21, MIR143, MIR145, and MIR205 and clinicopathologic features of esophageal squamous cell carcinoma
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DOI：
10.1111/j.1442-2050.2011.01177.x
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发表时间：
2011-09-01
影响因子：
2.6
通讯作者：
Takizawa, T.
中科院分区：
文献类型：
--
作者：
Akagi, I.;Miyashita, M.;Takizawa, T.
尽管microRNAs（miRNAs）的表达模式改变在各种癌症中的重要性是无可争议的，但是关于癌症相关miRNAs（MIR 21、MIR 143、MIR 144、MIR 145和MIR 205）在食管鳞状细胞癌（ESCC）中的临床病理学意义的信息很少。我们使用实时聚合酶链反应（PCR）检测了前体和成熟miRNA基因在ESCC中的表达水平。我们还研究了参与miRNA生物合成途径的加工元件（RNASEN，DGCR 8和DICER 1）的mRNA表达水平。进一步分析这5种miRNAs的表达水平与食管鳞癌患者临床病理参数的关系。成熟MIR 21和MIR 145在食管鳞癌中的表达水平明显高于正常食管上皮（P < 0.05）。食管鳞癌组织中MIR 21成熟/前体比例高于正常食管上皮组织（P < 0.05）。RNASEN在ESCC中的表达水平高于正常上皮（P < 0.05）。此外，这些miRNA的表达改变与ESCC患者的临床病理特征有关。成熟MIR 21和成熟MIR 205的高表达与食管鳞癌淋巴结转移有关（P < 0.05）。成熟MIR 143和成熟MIR 145的高表达与食管鳞癌的复发转移有关（P < 0.05）。这些发现可能意味着ESCC中miRNA的生物合成异常加速。分析miRNAs的表达水平将为ESCC患者的分期、预后和治疗提供有用的信息。
In spite of the undisputed importance of altered expression patterns of microRNAs (miRNAs) in various cancers, there is little information on the clinicopathologic significance of cancer-related miRNAs (MIR21, MIR143, MIR144, MIR145, and MIR205) in esophageal squamous cell carcinoma (ESCC). We examined the expression levels of the precursor and mature miRNA genes in ESCC using real-time polymerase chain reaction (PCR). We also investigated the mRNA expression levels of processing elements (RNASEN, DGCR8, and DICER1) that participate in miRNA-biogenesis pathway. Furthermore, we analyzed the relationships between the expression levels of these five miRNAs and the clinicopathologic parameters of ESCC patients. The expression levels of mature MIR21 and mature MIR145 were higher in ESCC than those in normal epithelium (P < 0.05). The mature/pre ratio of MIR21 in ESCC was higher than that in normal epithelium (P < 0.05). With regard to miRNA-processing elements, the expression level of RNASEN was higher in ESCC than in normal epithelium (P < 0.05). Furthermore, altered expression of these miRNAs was related to the clinicopathologic features of ESCC patients. The high expression of mature MIR21 and mature MIR205 was associated with lymph node positivity in ESCC patients (P < 0.05). The high levels of expression of mature MIR143 and mature MIR145 were associated with recurrence of metastasis in ESCC patients (P < 0.05). The findings may imply that miRNA biogenesis is aberrantly accelerated in ESCC. Analysis of the expression levels of miRNAs should provide useful information for evaluation of the staging, prognosis, and treatment of ESCC patients.