Cocoa procyanidins with different degrees of polymerization possess distinct activities in models of colonic inflammation.
Cocoa procyanidins with different degrees of polymerization possess distinct activities in models of colonic inflammation.
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DOI：
10.1016/j.jnutbio.2015.02.007
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发表时间：
2015-08
期刊：
影响因子：
--
通讯作者：
Neilson AP
中科院分区：
文献类型：
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作者：
Bitzer ZT;Glisan SL;Dorenkott MR;Goodrich KM;Ye L;O'Keefe SF;Lambert JD;Neilson AP
原花青素在饮食中可以从可可和葡萄等来源获得。原花青素的独特之处在于它们由重复的单体单元组成，可以以不同程度的聚合存在。聚合程度决定了原花青素的生物活性。然而，不能对原花青素结构和生物活性之间的关系进行概括，因为大小-活性关系似乎是系统依赖的。我们的目的是在体外筛选具有不同聚合程度的原花青素在结肠炎症模型中的抗炎活性。使用破坏膜完整性和人类结肠细胞炎症的细胞模型筛选单体、低聚和聚合可可原花青素组分。高分子量聚合物原花青素在保护膜完整性和减少炎症刺激时白细胞介素-8的分泌方面最有效。相反，低聚原花青素的效果最差。这些结果表明，聚合可可原花青素可能是预防肠道屏障功能丧失和上皮炎症最有效的，这是代谢性内毒素血症、炎症性肠病和结肠癌发病的关键步骤。因此，有必要进一步研究具有不同聚合度的可可原花青素，特别是高分子量原花青素的潜在健康保护益处。
Procyanidins are available in the diet from sources such as cocoa and grapes. Procyanidins are unique in that they are comprised of repeating monomeric units and can exist in various degrees of polymerization. The degree of polymerization plays a role in determining the biological activities of procyanidins. However, generalizations cannot be made regarding the correlation between procyanidin structure and bioactivity, because the size-activity relationship appears to be system-dependent. Our aim was to screen fractions of procyanidins with differing degrees of polymerization in vitro for anti-inflammatory activities in models of colonic inflammation. Monomeric, oligomeric, and polymeric cocoa procyanidin fractions were screened using cell models of disrupted membrane integrity and inflammation in human colon cells. High molecular weight polymeric procyanidins were the most effective at preserving membrane integrity and reducing secretion of interleukin-8 in response to inflammatory stimuli. Conversely, oligomeric procyanidins appeared to be the least effective. These results suggest that polymeric cocoa procyanidins may be the most effective for preventing loss of gut barrier function and epithelial inflammation, which are critical steps in the pathogenesis of metabolic endotoxemia, inflammatory bowel disease, and colon cancer. Therefore, further investigations of the potential health-protective benefits of cocoa procyanidins with distinct degrees of polymerization, particularly high molecular weight procyanidins, are warranted.