Interlaboratory comparison of assessments of Alzheimer disease-related lesions:: a study of the BrainNet Europe consortium
Interlaboratory comparison of assessments of Alzheimer disease-related lesions:: a study of the BrainNet Europe consortium
复制标题
DOI：
10.1097/01.jnen.0000229986.17548.27
复制
发表时间：
2006-08-01
影响因子：
3.2
通讯作者：
Kretzschmar, Hans
中科院分区：
文献类型：
--
作者：
Alafuzoff, Irina;Pikkarainen, Maria;Kretzschmar, Hans
这项实验室间研究评价了神经炎斑块和神经纤维缠结（NFT）（阿尔茨海默病的标志性病变）评估的重现性，并比较了BrainNet Europe中心之间的染色。为了减少评估中与地形相关的不一致性，我们使用了2 mm组织微阵列（TMA）技术。TMA区块包括取自21个石蜡区块的42个岩心样品。使用针对β-淀粉样蛋白（IHC/A β）和过度磷酸化tau（IHC/HPtau）的抗体，采用免疫组织化学（IHC）方法，在Bielschowsky和Gallyas银染色上进行评估。参与者之间的染色质量和评估不同，Bielschowsky最多样化（53%的中心染色良好/可接受），其次是Gallyas（57%的染色良好/可接受）和IHC/A β（71%的染色良好/可接受）。使用IHC/HPtau方法获得了最均匀的染色质量和评估（94%的中心染色良好/可接受）。使用的神经病理学诊断方案（建立阿尔茨海默病登记处联盟，Braak和Braak，以及国家老龄化研究所和里根[NIA-Reagan]研究所）显着影响了一致性，最高为NIA-Reagan（54%）建议。通过使用IHC/HPTau方法可视化NFT改善了该一致性。因此，IHC/HPTau方法可视化NFT和神经纤维瘤线程应被视为在未来的阿尔茨海默病诊断方案的选择方法。
This interlaboratory study evaluated the reproducibility of the assessments of neuritic plaques and neurofibrillary tangles (NFTs) - the hallmark lesions of Alzheimer disease-and compared the staining between the BrainNet Europe centers. To reduce the topography-related inconsistencies in assessments, we used a 2-mm tissue microarray (TMA) technique. The TMA block included 42 core samples taken from 21 paraffin blocks. The assessments were done on Bielschowsky and Gallyas silver stains using an immunohistochemical (IHC) method with antibodies directed to beta-amyloid (IHC/A beta) and hyperphosphorylated tau (IHC/HPtau). The staining quality and the assessments differed between the participants, being most diverse with Bielschowsky (good/acceptable stain in 53% of centers) followed by Gallyas (good/acceptable stain in 57%) and IHC/A beta (good/acceptable stain in 71%). The most uniform staining quality and assessment was obtained with the IHC/HPtau method (good/ acceptable stain in 94% of centers). The neuropathologic diagnostic protocol (Consortium to Establish a Registry for Alzheimer Disease, Braak and Braak, and the National Institute of Aging and Reagan [NIA-Reagan] Institute) that was used significantly influenced the agreement, being highest with NIA-Reagan (54%) recommendations. This agreement was improved by visualization of NFTs using the IHC/HPtau method. Therefore, the IHC/HPtau methodology to visualize NFTs and neuropil threads should be considered as a method of choice in a future diagnostic protocol for Alzheimer disease.